我会尝试这样的方法。

    try:
            f = open(filePath)
    except IOError:
            print "No such file: %s" % filePath
            raw_input("Press Enter to close window")
    try:
            lines = f.readlines()
            while True:
                    line = f.readline()
                    try:
                            if not line:
                                    time.sleep(1)
                            else:
                                    functionThatAnalisesTheLine(line)
                    except Exception, e:
                            # handle the exception somehow (for example, log the trace) and raise the same exception again
                            raw_input("Press Enter to close window")
                            raise e
    finally:
            f.close()

循环检查自上次读取文件以来是否有新的行——如果有，则将其读取并传递给functionThatAnalisesTheLine函数。如果不是，脚本等待1秒并重试该进程。

我不知道任何Windows特有的函数。您可以尝试每秒钟/分钟/小时获取文件的MD5哈希值(取决于您需要它的速度)，并将其与最后的哈希值进行比较。当它不同时，您知道文件已被更改，并读取最新的行。

因为我已经全局安装了它，所以我最喜欢的方法是使用nodemon。如果你的源代码在src中，你的入口点是src/app.py，那么就像这样简单:

nodemon -w 'src/**' -e py,html --exec python src/app.py

．.． 其中-e py,html允许您控制要监视更改的文件类型。

为了用轮询和最小依赖来观察单个文件，下面是一个完整的例子，基于Deestan的回答(上面):

import os
import sys 
import time

class Watcher(object):
    running = True
    refresh_delay_secs = 1

    # Constructor
    def __init__(self, watch_file, call_func_on_change=None, *args, **kwargs):
        self._cached_stamp = 0
        self.filename = watch_file
        self.call_func_on_change = call_func_on_change
        self.args = args
        self.kwargs = kwargs

    # Look for changes
    def look(self):
        stamp = os.stat(self.filename).st_mtime
        if stamp != self._cached_stamp:
            self._cached_stamp = stamp
            # File has changed, so do something...
            print('File changed')
            if self.call_func_on_change is not None:
                self.call_func_on_change(*self.args, **self.kwargs)

    # Keep watching in a loop        
    def watch(self):
        while self.running: 
            try: 
                # Look for changes
                time.sleep(self.refresh_delay_secs) 
                self.look() 
            except KeyboardInterrupt: 
                print('\nDone') 
                break 
            except FileNotFoundError:
                # Action on file not found
                pass
            except: 
                print('Unhandled error: %s' % sys.exc_info()[0])

# Call this function each time a change happens
def custom_action(text):
    print(text)

watch_file = 'my_file.txt'

# watcher = Watcher(watch_file)  # simple
watcher = Watcher(watch_file, custom_action, text='yes, changed')  # also call custom action function
watcher.watch()  # start the watch going

ACTIONS = {
  1 : "Created",
  2 : "Deleted",
  3 : "Updated",
  4 : "Renamed from something",
  5 : "Renamed to something"
}
FILE_LIST_DIRECTORY = 0x0001

class myThread (threading.Thread):
    def __init__(self, threadID, fileName, directory, origin):
        threading.Thread.__init__(self)
        self.threadID = threadID
        self.fileName = fileName
        self.daemon = True
        self.dir = directory
        self.originalFile = origin
    def run(self):
        startMonitor(self.fileName, self.dir, self.originalFile)

def startMonitor(fileMonitoring,dirPath,originalFile):
    hDir = win32file.CreateFile (
        dirPath,
        FILE_LIST_DIRECTORY,
        win32con.FILE_SHARE_READ | win32con.FILE_SHARE_WRITE,
        None,
        win32con.OPEN_EXISTING,
        win32con.FILE_FLAG_BACKUP_SEMANTICS,
        None
    )
    # Wait for new data and call ProcessNewData for each new chunk that's
    # written
    while 1:
        # Wait for a change to occur
        results = win32file.ReadDirectoryChangesW (
            hDir,
            1024,
            False,
            win32con.FILE_NOTIFY_CHANGE_LAST_WRITE,
            None,
            None
        )
        # For each change, check to see if it's updating the file we're
        # interested in
        for action, file_M in results:
            full_filename = os.path.join (dirPath, file_M)
            #print file, ACTIONS.get (action, "Unknown")
            if len(full_filename) == len(fileMonitoring) and action == 3:
                #copy to main file
                ...

如果您想要一个多平台解决方案，那么请检查QFileSystemWatcher。 下面是一个示例代码(未消毒):

from PyQt4 import QtCore

@QtCore.pyqtSlot(str)
def directory_changed(path):
    print('Directory Changed!!!')

@QtCore.pyqtSlot(str)
def file_changed(path):
    print('File Changed!!!')

fs_watcher = QtCore.QFileSystemWatcher(['/path/to/files_1', '/path/to/files_2', '/path/to/files_3'])

fs_watcher.connect(fs_watcher, QtCore.SIGNAL('directoryChanged(QString)'), directory_changed)
fs_watcher.connect(fs_watcher, QtCore.SIGNAL('fileChanged(QString)'), file_changed)