为了用轮询和最小依赖来观察单个文件，下面是一个完整的例子，基于Deestan的回答(上面):

import os
import sys 
import time

class Watcher(object):
    running = True
    refresh_delay_secs = 1

    # Constructor
    def __init__(self, watch_file, call_func_on_change=None, *args, **kwargs):
        self._cached_stamp = 0
        self.filename = watch_file
        self.call_func_on_change = call_func_on_change
        self.args = args
        self.kwargs = kwargs

    # Look for changes
    def look(self):
        stamp = os.stat(self.filename).st_mtime
        if stamp != self._cached_stamp:
            self._cached_stamp = stamp
            # File has changed, so do something...
            print('File changed')
            if self.call_func_on_change is not None:
                self.call_func_on_change(*self.args, **self.kwargs)

    # Keep watching in a loop        
    def watch(self):
        while self.running: 
            try: 
                # Look for changes
                time.sleep(self.refresh_delay_secs) 
                self.look() 
            except KeyboardInterrupt: 
                print('\nDone') 
                break 
            except FileNotFoundError:
                # Action on file not found
                pass
            except: 
                print('Unhandled error: %s' % sys.exc_info()[0])

# Call this function each time a change happens
def custom_action(text):
    print(text)

watch_file = 'my_file.txt'

# watcher = Watcher(watch_file)  # simple
watcher = Watcher(watch_file, custom_action, text='yes, changed')  # also call custom action function
watcher.watch()  # start the watch going

下面是Kender代码的简化版本，它似乎做了同样的技巧，并且没有导入整个文件:

# Check file for new data.

import time

f = open(r'c:\temp\test.txt', 'r')

while True:

    line = f.readline()
    if not line:
        time.sleep(1)
        print 'Nothing New'
    else:
        print 'Call Function: ', line

我不知道任何Windows特有的函数。您可以尝试每秒钟/分钟/小时获取文件的MD5哈希值(取决于您需要它的速度)，并将其与最后的哈希值进行比较。当它不同时，您知道文件已被更改，并读取最新的行。

我会尝试这样的方法。

    try:
            f = open(filePath)
    except IOError:
            print "No such file: %s" % filePath
            raw_input("Press Enter to close window")
    try:
            lines = f.readlines()
            while True:
                    line = f.readline()
                    try:
                            if not line:
                                    time.sleep(1)
                            else:
                                    functionThatAnalisesTheLine(line)
                    except Exception, e:
                            # handle the exception somehow (for example, log the trace) and raise the same exception again
                            raw_input("Press Enter to close window")
                            raise e
    finally:
            f.close()

循环检查自上次读取文件以来是否有新的行——如果有，则将其读取并传递给functionThatAnalisesTheLine函数。如果不是，脚本等待1秒并重试该进程。

你试过用看门狗吗?

Python API库和shell实用程序来监视文件系统事件。 目录监视变得很容易 一个跨平台的API。 一个shell工具，用于在目录更改时运行命令。 用一个简单的例子开始快速入门…

下面是一个示例，用于观察每秒写入不超过一行但通常要少得多的输入文件。目标是将最后一行(最近的写入)追加到指定的输出文件。我从我的一个项目中复制了这个，只是删除了所有不相关的行。你必须填写或修改缺失的符号。

from PyQt5.QtCore import QFileSystemWatcher, QSettings, QThread
from ui_main_window import Ui_MainWindow   # Qt Creator gen'd 

class MainWindow(QMainWindow, Ui_MainWindow):
    def __init__(self, parent=None):
        QMainWindow.__init__(self, parent)
        Ui_MainWindow.__init__(self)
        self._fileWatcher = QFileSystemWatcher()
        self._fileWatcher.fileChanged.connect(self.fileChanged)

    def fileChanged(self, filepath):
        QThread.msleep(300)    # Reqd on some machines, give chance for write to complete
        # ^^ About to test this, may need more sophisticated solution
        with open(filepath) as file:
            lastLine = list(file)[-1]
        destPath = self._filemap[filepath]['dest file']
        with open(destPath, 'a') as out_file:               # a= append
            out_file.writelines([lastLine])

当然，并不是严格要求包含QMainWindow类。您可以单独使用QFileSystemWatcher。