为了用轮询和最小依赖来观察单个文件，下面是一个完整的例子，基于Deestan的回答(上面):

import os
import sys 
import time

class Watcher(object):
    running = True
    refresh_delay_secs = 1

    # Constructor
    def __init__(self, watch_file, call_func_on_change=None, *args, **kwargs):
        self._cached_stamp = 0
        self.filename = watch_file
        self.call_func_on_change = call_func_on_change
        self.args = args
        self.kwargs = kwargs

    # Look for changes
    def look(self):
        stamp = os.stat(self.filename).st_mtime
        if stamp != self._cached_stamp:
            self._cached_stamp = stamp
            # File has changed, so do something...
            print('File changed')
            if self.call_func_on_change is not None:
                self.call_func_on_change(*self.args, **self.kwargs)

    # Keep watching in a loop        
    def watch(self):
        while self.running: 
            try: 
                # Look for changes
                time.sleep(self.refresh_delay_secs) 
                self.look() 
            except KeyboardInterrupt: 
                print('\nDone') 
                break 
            except FileNotFoundError:
                # Action on file not found
                pass
            except: 
                print('Unhandled error: %s' % sys.exc_info()[0])

# Call this function each time a change happens
def custom_action(text):
    print(text)

watch_file = 'my_file.txt'

# watcher = Watcher(watch_file)  # simple
watcher = Watcher(watch_file, custom_action, text='yes, changed')  # also call custom action function
watcher.watch()  # start the watch going

我不知道任何Windows特有的函数。您可以尝试每秒钟/分钟/小时获取文件的MD5哈希值(取决于您需要它的速度)，并将其与最后的哈希值进行比较。当它不同时，您知道文件已被更改，并读取最新的行。

如果您想要一个多平台解决方案，那么请检查QFileSystemWatcher。 下面是一个示例代码(未消毒):

from PyQt4 import QtCore

@QtCore.pyqtSlot(str)
def directory_changed(path):
    print('Directory Changed!!!')

@QtCore.pyqtSlot(str)
def file_changed(path):
    print('File Changed!!!')

fs_watcher = QtCore.QFileSystemWatcher(['/path/to/files_1', '/path/to/files_2', '/path/to/files_3'])

fs_watcher.connect(fs_watcher, QtCore.SIGNAL('directoryChanged(QString)'), directory_changed)
fs_watcher.connect(fs_watcher, QtCore.SIGNAL('fileChanged(QString)'), file_changed)

下面是一个示例，用于观察每秒写入不超过一行但通常要少得多的输入文件。目标是将最后一行(最近的写入)追加到指定的输出文件。我从我的一个项目中复制了这个，只是删除了所有不相关的行。你必须填写或修改缺失的符号。

from PyQt5.QtCore import QFileSystemWatcher, QSettings, QThread
from ui_main_window import Ui_MainWindow   # Qt Creator gen'd 

class MainWindow(QMainWindow, Ui_MainWindow):
    def __init__(self, parent=None):
        QMainWindow.__init__(self, parent)
        Ui_MainWindow.__init__(self)
        self._fileWatcher = QFileSystemWatcher()
        self._fileWatcher.fileChanged.connect(self.fileChanged)

    def fileChanged(self, filepath):
        QThread.msleep(300)    # Reqd on some machines, give chance for write to complete
        # ^^ About to test this, may need more sophisticated solution
        with open(filepath) as file:
            lastLine = list(file)[-1]
        destPath = self._filemap[filepath]['dest file']
        with open(destPath, 'a') as out_file:               # a= append
            out_file.writelines([lastLine])

当然，并不是严格要求包含QMainWindow类。您可以单独使用QFileSystemWatcher。

在对蒂姆·戈尔登的剧本进行了一些修改后，我有了以下似乎工作得相当不错的内容:

import os

import win32file
import win32con

path_to_watch = "." # look at the current directory
file_to_watch = "test.txt" # look for changes to a file called test.txt

def ProcessNewData( newData ):
    print "Text added: %s"%newData

# Set up the bits we'll need for output
ACTIONS = {
  1 : "Created",
  2 : "Deleted",
  3 : "Updated",
  4 : "Renamed from something",
  5 : "Renamed to something"
}
FILE_LIST_DIRECTORY = 0x0001
hDir = win32file.CreateFile (
  path_to_watch,
  FILE_LIST_DIRECTORY,
  win32con.FILE_SHARE_READ | win32con.FILE_SHARE_WRITE,
  None,
  win32con.OPEN_EXISTING,
  win32con.FILE_FLAG_BACKUP_SEMANTICS,
  None
)

# Open the file we're interested in
a = open(file_to_watch, "r")

# Throw away any exising log data
a.read()

# Wait for new data and call ProcessNewData for each new chunk that's written
while 1:
  # Wait for a change to occur
  results = win32file.ReadDirectoryChangesW (
    hDir,
    1024,
    False,
    win32con.FILE_NOTIFY_CHANGE_LAST_WRITE,
    None,
    None
  )

  # For each change, check to see if it's updating the file we're interested in
  for action, file in results:
    full_filename = os.path.join (path_to_watch, file)
    #print file, ACTIONS.get (action, "Unknown")
    if file == file_to_watch:
        newText = a.read()
        if newText != "":
            ProcessNewData( newText )

它可能需要进行更多的错误检查，但对于简单地观察日志文件并在将其输出到屏幕之前对其进行一些处理，这样做效果很好。

谢谢大家的投入-很棒的东西!

我会尝试这样的方法。

    try:
            f = open(filePath)
    except IOError:
            print "No such file: %s" % filePath
            raw_input("Press Enter to close window")
    try:
            lines = f.readlines()
            while True:
                    line = f.readline()
                    try:
                            if not line:
                                    time.sleep(1)
                            else:
                                    functionThatAnalisesTheLine(line)
                    except Exception, e:
                            # handle the exception somehow (for example, log the trace) and raise the same exception again
                            raw_input("Press Enter to close window")
                            raise e
    finally:
            f.close()

循环检查自上次读取文件以来是否有新的行——如果有，则将其读取并传递给functionThatAnalisesTheLine函数。如果不是，脚本等待1秒并重试该进程。