如果文件夹不在图像文件夹或其他文件夹下，请使用此代码

string subPath = HttpContext.Current.Server.MapPath(@"~/Images/RequisitionBarCode/");

bool exists = System.IO.Directory.Exists(subPath);
if(!exists)
    System.IO.Directory.CreateDirectory(subPath);

string path = HttpContext.Current.Server.MapPath(@"~/Images/RequisitionBarCode/" + OrderId + ".png");

使用下面的代码按如何使用文件上传服务器控件动态创建文件夹?：

string subPath ="ImagesPath"; // Your code goes here

bool exists = System.IO.Directory.Exists(Server.MapPath(subPath));

if(!exists)
    System.IO.Directory.CreateDirectory(Server.MapPath(subPath));

一种奇特的方法是使用您想要的方法扩展FileUpload。

添加:

public static class FileUploadExtension
{
    public static void SaveAs(this FileUpload, string destination, bool autoCreateDirectory) { 

        if (autoCreateDirectory)
        {
            var destinationDirectory = new DirectoryInfo(Path.GetDirectoryName(destination));

            if (!destinationDirectory.Exists)
                destinationDirectory.Create();
        }

        file.SaveAs(destination);
    }
}

然后使用它:

FileUpload file;
...
file.SaveAs(path,true);

如果路径还不存在，可以使用如下方法创建路径:

using System.IO;

private void CreateIfMissing(string path)
{
  bool folderExists = Directory.Exists(Server.MapPath(path));
  if (!folderExists)
    Directory.CreateDirectory(Server.MapPath(path));
}

如果文件夹不在图像文件夹或其他文件夹下，请使用此代码

string subPath = HttpContext.Current.Server.MapPath(@"~/Images/RequisitionBarCode/");

bool exists = System.IO.Directory.Exists(subPath);
if(!exists)
    System.IO.Directory.CreateDirectory(subPath);

string path = HttpContext.Current.Server.MapPath(@"~/Images/RequisitionBarCode/" + OrderId + ".png");

从多个答案衍生/组合而来，对我来说，实现它就像这样简单:

public void Init()
{
    String platypusDir = @"C:\platypus";
    CreateDirectoryIfDoesNotExist(platypusDir);
}

private void CreateDirectoryIfDoesNotExist(string dirName)
{
    System.IO.Directory.CreateDirectory(dirName);
}