/*InputStream class_InputStream = null;
I am reading class from DB 
class_InputStream = rs.getBinaryStream(1);
Your Input stream could be from any source
*/
int thisLine;
ByteArrayOutputStream bos = new ByteArrayOutputStream();
while ((thisLine = class_InputStream.read()) != -1) {
    bos.write(thisLine);
}
bos.flush();
byte [] yourBytes = bos.toByteArray();

/*Don't forget in the finally block to close ByteArrayOutputStream & InputStream
 In my case the IS is from resultset so just closing the rs will do it*/

if (bos != null){
    bos.close();
}

Input Stream is ...
ByteArrayOutputStream bos = new ByteArrayOutputStream();
int next = in.read();
while (next > -1) {
    bos.write(next);
    next = in.read();
}
bos.flush();
byte[] result = bos.toByteArray();
bos.close();

Java 8方式(感谢BufferedReader和Adam Bien)

private static byte[] readFully(InputStream input) throws IOException {
    try (BufferedReader buffer = new BufferedReader(new InputStreamReader(input))) {
        return buffer.lines().collect(Collectors.joining("\n")).getBytes(<charset_can_be_specified>);
    }
}

注意，这个解决方案删除回车符('\r')，可能是不合适的。

下面是一个优化的版本，尽量避免复制数据字节:

private static byte[] loadStream (InputStream stream) throws IOException {
   int available = stream.available();
   int expectedSize = available > 0 ? available : -1;
   return loadStream(stream, expectedSize);
}

private static byte[] loadStream (InputStream stream, int expectedSize) throws IOException {
   int basicBufferSize = 0x4000;
   int initialBufferSize = (expectedSize >= 0) ? expectedSize : basicBufferSize;
   byte[] buf = new byte[initialBufferSize];
   int pos = 0;
   while (true) {
      if (pos == buf.length) {
         int readAhead = -1;
         if (pos == expectedSize) {
            readAhead = stream.read();       // test whether EOF is at expectedSize
            if (readAhead == -1) {
               return buf;
            }
         }
         int newBufferSize = Math.max(2 * buf.length, basicBufferSize);
         buf = Arrays.copyOf(buf, newBufferSize);
         if (readAhead != -1) {
            buf[pos++] = (byte)readAhead;
         }
      }
      int len = stream.read(buf, pos, buf.length - pos);
      if (len < 0) {
         return Arrays.copyOf(buf, pos);
      }
      pos += len;
   }
}

使用普通Java的DataInputStream和它的readFully方法(至少从Java 1.4开始存在):

...
byte[] bytes = new byte[(int) file.length()];
DataInputStream dis = new DataInputStream(new FileInputStream(file));
dis.readFully(bytes);
...

这个方法还有其他一些形式，但在这个用例中，我一直使用这个方法。

如果您不想使用Apache common -io库，则此代码片段取自sun.misc.IOUtils类。它的速度几乎是使用ByteBuffers的普通实现的两倍:

public static byte[] readFully(InputStream is, int length, boolean readAll)
        throws IOException {
    byte[] output = {};
    if (length == -1) length = Integer.MAX_VALUE;
    int pos = 0;
    while (pos < length) {
        int bytesToRead;
        if (pos >= output.length) { // Only expand when there's no room
            bytesToRead = Math.min(length - pos, output.length + 1024);
            if (output.length < pos + bytesToRead) {
                output = Arrays.copyOf(output, pos + bytesToRead);
            }
        } else {
            bytesToRead = output.length - pos;
        }
        int cc = is.read(output, pos, bytesToRead);
        if (cc < 0) {
            if (readAll && length != Integer.MAX_VALUE) {
                throw new EOFException("Detect premature EOF");
            } else {
                if (output.length != pos) {
                    output = Arrays.copyOf(output, pos);
                }
                break;
            }
        }
        pos += cc;
    }
    return output;
}