Java 7及以上版本:

import sun.misc.IOUtils;
...
InputStream in = ...;
byte[] buf = IOUtils.readFully(in, -1, false);

/*InputStream class_InputStream = null;
I am reading class from DB 
class_InputStream = rs.getBinaryStream(1);
Your Input stream could be from any source
*/
int thisLine;
ByteArrayOutputStream bos = new ByteArrayOutputStream();
while ((thisLine = class_InputStream.read()) != -1) {
    bos.write(thisLine);
}
bos.flush();
byte [] yourBytes = bos.toByteArray();

/*Don't forget in the finally block to close ByteArrayOutputStream & InputStream
 In my case the IS is from resultset so just closing the rs will do it*/

if (bos != null){
    bos.close();
}

Input Stream is ...
ByteArrayOutputStream bos = new ByteArrayOutputStream();
int next = in.read();
while (next > -1) {
    bos.write(next);
    next = in.read();
}
bos.flush();
byte[] result = bos.toByteArray();
bos.close();

请参阅InputStream.available()文档:

It is particularly important to realize that you must not use this method to size a container and assume that you can read the entirety of the stream without needing to resize the container. Such callers should probably write everything they read to a ByteArrayOutputStream and convert that to a byte array. Alternatively, if you're reading from a file, File.length returns the current length of the file (though assuming the file's length can't change may be incorrect, reading a file is inherently racy).

我知道已经太迟了，但我认为这里有更清晰的解决方案，更易于阅读……

/**
 * method converts {@link InputStream} Object into byte[] array.
 * 
 * @param stream the {@link InputStream} Object.
 * @return the byte[] array representation of received {@link InputStream} Object.
 * @throws IOException if an error occurs.
 */
public static byte[] streamToByteArray(InputStream stream) throws IOException {

    byte[] buffer = new byte[1024];
    ByteArrayOutputStream os = new ByteArrayOutputStream();

    int line = 0;
    // read bytes from stream, and store them in buffer
    while ((line = stream.read(buffer)) != -1) {
        // Writes bytes from byte array (buffer) into output stream.
        os.write(buffer, 0, line);
    }
    stream.close();
    os.flush();
    os.close();
    return os.toByteArray();
}

使用普通Java的DataInputStream和它的readFully方法(至少从Java 1.4开始存在):

...
byte[] bytes = new byte[(int) file.length()];
DataInputStream dis = new DataInputStream(new FileInputStream(file));
dis.readFully(bytes);
...

这个方法还有其他一些形式，但在这个用例中，我一直使用这个方法。