file = '/path/to/file_to_be_read.txt'
with open(file) as f:
    print f.readlines()[26]
    print f.readlines()[30]

使用with语句打开文件，打印第26行和第30行，然后关闭文件。简单!

如果你想看第7行

line = open("file.txt", "r").readlines()[7]

我认为这行得通

 open_file1 = open("E:\\test.txt",'r')
 read_it1 = open_file1.read()
 myline1 = []
 for line1 in read_it1.splitlines():
 myline1.append(line1)
 print myline1[0]

如果你不介意导入，那么fileinput确实是你需要的(这是你可以读取当前行的行号)

您可以使用一种最简单的逻辑将字符串拆分为数组或List。

f = open('filepath')
r = f.read()
s = r.split("\n")
n = [linenumber1, linenumber2] # [26, 29] in your 
                               #case
for x in n:
  print(s[x-1])
f.close()

这个怎么样:

>>> with open('a', 'r') as fin: lines = fin.readlines()
>>> for i, line in enumerate(lines):
      if i > 30: break
      if i == 26: dox()
      if i == 30: doy()