你可以用已经有人提到过的语法很简单地做到这一点，但这是迄今为止最简单的方法:

inputFile = open("lineNumbers.txt", "r")
lines = inputFile.readlines()
print (lines[0])
print (lines[2])

您可以使用一种最简单的逻辑将字符串拆分为数组或List。

f = open('filepath')
r = f.read()
s = r.split("\n")
n = [linenumber1, linenumber2] # [26, 29] in your 
                               #case
for x in n:
  print(s[x-1])
f.close()

如果您的大型文本文件是严格结构良好的(意味着每一行都有相同的长度l)，您可以使用第n行

with open(file) as f:
    f.seek(n*l)
    line = f.readline() 
    last_pos = f.tell()

这只适用于具有相同长度的文件!

def getitems(iterable, items):
  items = list(items) # get a list from any iterable and make our own copy
                      # since we modify it
  if items:
    items.sort()
    for n, v in enumerate(iterable):
      if n == items[0]:
        yield v
        items.pop(0)
        if not items:
          break

print list(getitems(open("/usr/share/dict/words"), [25, 29]))
# ['Abelson\n', 'Abernathy\n']
# note that index 25 is the 26th item

从某一行读出:

n = 4   # for reading from 5th line
with open("write.txt",'r') as t:
     for i,line in enumerate(t):
         if i >= n:             # i == n-1 for nth line
            print(line)

这个怎么样:

>>> with open('a', 'r') as fin: lines = fin.readlines()
>>> for i, line in enumerate(lines):
      if i > 30: break
      if i == 26: dox()
      if i == 30: doy()