现有的解决方案不适合我(详情见下文)。最后成功的是使用read(1)实现readline(基于这个答案)。后者不阻塞:

from subprocess import Popen, PIPE
from threading import Thread
def process_output(myprocess): #output-consuming thread
    nextline = None
    buf = ''
    while True:
        #--- extract line using read(1)
        out = myprocess.stdout.read(1)
        if out == '' and myprocess.poll() != None: break
        if out != '':
            buf += out
            if out == '\n':
                nextline = buf
                buf = ''
        if not nextline: continue
        line = nextline
        nextline = None

        #--- do whatever you want with line here
        print 'Line is:', line
    myprocess.stdout.close()

myprocess = Popen('myprogram.exe', stdout=PIPE) #output-producing process
p1 = Thread(target=process_output, args=(myprocess,)) #output-consuming thread
p1.daemon = True
p1.start()

#--- do whatever here and then kill process and thread if needed
if myprocess.poll() == None: #kill process; will automatically stop thread
    myprocess.kill()
    myprocess.wait()
if p1 and p1.is_alive(): #wait for thread to finish
    p1.join()

为什么现有的解决方案不起作用:

Solutions that require readline (including the Queue based ones) always block. It is difficult (impossible?) to kill the thread that executes readline. It only gets killed when the process that created it finishes, but not when the output-producing process is killed. Mixing low-level fcntl with high-level readline calls may not work properly as anonnn has pointed out. Using select.poll() is neat, but doesn't work on Windows according to python docs. Using third-party libraries seems overkill for this task and adds additional dependencies.

为什么要打扰线程和队列? 与readline()不同，BufferedReader.read1()不会阻塞等待\r\n，如果有任何输出进入，它会尽快返回。

#!/usr/bin/python
from subprocess import Popen, PIPE, STDOUT
import io

def __main__():
    try:
        p = Popen( ["ping", "-n", "3", "127.0.0.1"], stdin=PIPE, stdout=PIPE, stderr=STDOUT )
    except: print("Popen failed"); quit()
    sout = io.open(p.stdout.fileno(), 'rb', closefd=False)
    while True:
        buf = sout.read1(1024)
        if len(buf) == 0: break
        print buf,

if __name__ == '__main__':
    __main__()

You can do this really easily in Twisted. Depending upon your existing code base, this might not be that easy to use, but if you are building a twisted application, then things like this become almost trivial. You create a ProcessProtocol class, and override the outReceived() method. Twisted (depending upon the reactor used) is usually just a big select() loop with callbacks installed to handle data from different file descriptors (often network sockets). So the outReceived() method is simply installing a callback for handling data coming from STDOUT. A simple example demonstrating this behavior is as follows:

from twisted.internet import protocol, reactor

class MyProcessProtocol(protocol.ProcessProtocol):

    def outReceived(self, data):
        print data

proc = MyProcessProtocol()
reactor.spawnProcess(proc, './myprogram', ['./myprogram', 'arg1', 'arg2', 'arg3'])
reactor.run()

Twisted文档在这方面有一些很好的信息。

如果您围绕Twisted构建整个应用程序，它可以与其他进程(本地或远程)进行异步通信，就像这样非常优雅。另一方面，如果您的程序不是构建在Twisted之上，那么这真的不会有多大帮助。希望这能对其他读者有所帮助，即使它不适用于您的特定应用程序。

Python 3.4为异步IO引入了新的临时API——asyncio模块。

该方法类似于@Bryan Ward的twisted-based回答——定义一个协议，一旦数据准备好，就调用它的方法:

#!/usr/bin/env python3
import asyncio
import os

class SubprocessProtocol(asyncio.SubprocessProtocol):
    def pipe_data_received(self, fd, data):
        if fd == 1: # got stdout data (bytes)
            print(data)

    def connection_lost(self, exc):
        loop.stop() # end loop.run_forever()

if os.name == 'nt':
    loop = asyncio.ProactorEventLoop() # for subprocess' pipes on Windows
    asyncio.set_event_loop(loop)
else:
    loop = asyncio.get_event_loop()
try:
    loop.run_until_complete(loop.subprocess_exec(SubprocessProtocol, 
        "myprogram.exe", "arg1", "arg2"))
    loop.run_forever()
finally:
    loop.close()

请参阅文档中的“Subprocess”。

有一个高级接口asyncio.create_subprocess_exec()，它返回允许使用StreamReader.readline()协程异步读取一行的Process对象 (使用async/await Python 3.5+语法):

#!/usr/bin/env python3.5
import asyncio
import locale
import sys
from asyncio.subprocess import PIPE
from contextlib import closing

async def readline_and_kill(*args):
    # start child process
    process = await asyncio.create_subprocess_exec(*args, stdout=PIPE)

    # read line (sequence of bytes ending with b'\n') asynchronously
    async for line in process.stdout:
        print("got line:", line.decode(locale.getpreferredencoding(False)))
        break
    process.kill()
    return await process.wait() # wait for the child process to exit


if sys.platform == "win32":
    loop = asyncio.ProactorEventLoop()
    asyncio.set_event_loop(loop)
else:
    loop = asyncio.get_event_loop()

with closing(loop):
    sys.exit(loop.run_until_complete(readline_and_kill(
        "myprogram.exe", "arg1", "arg2")))

Readline_and_kill()执行以下任务:

启动子进程，将其标准输出重定向到管道 异步从子进程的stdout中读取一行 杀子流程 等待它退出

如果需要，每个步骤都可以被超时秒限制。

一种解决方案是让另一个进程执行对该进程的读取，或者创建一个带有超时的进程线程。

这是一个超时函数的线程版本:

http://code.activestate.com/recipes/473878/

但是，是否需要在stdout传入时读取它? 另一种解决方案可能是将输出转储到一个文件中，然后使用p.t wait()等待进程完成。

f = open('myprogram_output.txt','w')
p = subprocess.Popen('myprogram.exe', stdout=f)
p.wait()
f.close()


str = open('myprogram_output.txt','r').read()

尝试wexpect，它是pexpect的windows替代品。

import wexpect

p = wexpect.spawn('myprogram.exe')
p.stdout.readline('.')               // regex pattern of any character
output_str = p.after()