datetime
t = datetime.datetime.now()
ms = '%s.%i' % (t.strftime('%H:%M:%S'), t.microsecond/1000)
print(ms)
14:44:37.134

datetime
t = datetime.datetime.now()
ms = '%s.%i' % (t.strftime('%H:%M:%S'), t.microsecond/1000)
print(ms)
14:44:37.134

在python 3.6及以上版本中使用python f-strings:

from datetime import datetime, timezone

dt = datetime.now(timezone.utc)

print(f"{dt:%Y-%m-%d %H:%M:%S}.{dt.microsecond // 1000:03d}")

特定于格式化毫秒的代码是:

{dt.microsecond // 1000:03d}

格式字符串{:03d}和微秒到毫秒的转换// 1000来自https://github.com/python/cpython/blob/master/Lib/datetime.py中的def _format_time，用于datetime.datetime.isoformat()。

@Cabbi提出了一个问题，在一些系统上(带有Python 2.7的Windows)，微秒格式%f可能会错误地给出“0”，所以简单地修改最后三个字符是不可移植的。这样的系统不遵循文档中指定的行为:

下面的代码小心地以毫秒为单位格式化时间戳:

>>> from datetime import datetime
>>> (dt, micro) = datetime.utcnow().strftime('%Y-%m-%d %H:%M:%S.%f').split('.')
>>> "%s.%03d" % (dt, int(micro) / 1000)
'2016-02-26 04:37:53.133'

为了得到OP想要的准确输出，我们必须去掉标点符号:

>>> from datetime import datetime
>>> (dt, micro) = datetime.utcnow().strftime('%Y%m%d%H%M%S.%f').split('.')
>>> "%s%03d" % (dt, int(micro) / 1000)
'20160226043839901'

import datetime

# convert string into date time format.

str_date = '2016-10-06 15:14:54.322989'
d_date = datetime.datetime.strptime(str_date , '%Y-%m-%d %H:%M:%S.%f')
print(d_date)
print(type(d_date)) # check d_date type.


# convert date time to regular format.

reg_format_date = d_date.strftime("%d %B %Y %I:%M:%S %p")
print(reg_format_date)

# some other date formats.
reg_format_date = d_date.strftime("%Y-%m-%d %I:%M:%S %p")
print(reg_format_date)
reg_format_date = d_date.strftime("%Y-%m-%d %H:%M:%S")
print(reg_format_date)

<<<<<<输出>>>>>>>

2016-10-06 15:14:54.322989    
<class 'datetime.datetime'>    
06 October 2016 03:14:54 PM    
2016-10-06 03:14:54 PM    
2016-10-06 15:14:54

python -c "from datetime import datetime; print str(datetime.now())[:-3]"
2017-02-09 10:06:37.006