我使用monotouch，所以名称和代码会有点不同，但你可以通过确保集合视图的宽度等于(x *单元格宽度)+ (x-1) * MinimumSpacing与x =每行单元格的数量来做到这一点。

只需要根据MinimumInteritemSpacing和Cell的宽度执行以下步骤

1)我们根据单元格大小+当前插入+最小间距计算每行项目的数量

float currentTotalWidth = CollectionView.Frame.Width - Layout.SectionInset.Left - Layout.SectionInset.Right (Layout = flowlayout)
int amountOfCellsPerRow = (currentTotalWidth + MinimumSpacing) / (cell width + MinimumSpacing)

2)现在你有了所有信息来计算集合视图的预期宽度

float totalWidth =(amountOfCellsPerRow * cell width) + (amountOfCellsPerRow-1) * MinimumSpacing

3)所以当前宽度与预期宽度之差为

float difference = currentTotalWidth - totalWidth;

4)现在调整insets(在本例中，我们将其添加到右侧，因此集合视图的左侧位置保持不变

Layout.SectionInset.Right = Layout.SectionInset.Right + difference;

使用水平流布局，我也得到了10点之间的单元格间距。为了移除间距，我需要将minimumlinespespaces和minimumInterItemSpacing设置为零。

UICollectionViewFlowLayout *flow = [[UICollectionViewFlowLayout alloc] init];
flow.itemSize = CGSizeMake(cellWidth, cellHeight);
flow.scrollDirection = UICollectionViewScrollDirectionHorizontal;
flow.minimumInteritemSpacing = 0;
flow.minimumLineSpacing = 0;

此外，如果所有单元格大小相同，直接在流布局上设置属性比使用委托方法更简单、更有效。

投票的答案(以及快速版本)有一个小问题:右边会有一个很大的空格。

这是因为流布局是定制的，以使单元格间距精确，具有浮动左行为。

我的解决方案是操纵部分插入，使部分是对齐中心，但间距是完全指定的。

在下面的截图中，项目/行间距正好是8pt，而左右插入部分将大于8pt(使其居中对齐):

这样的Swift代码:

private let minItemSpacing: CGFloat = 8
private let itemWidth: CGFloat      = 100
private let headerHeight: CGFloat   = 32

override func viewDidLayoutSubviews() {
    super.viewDidLayoutSubviews()

    // Create our custom flow layout that evenly space out the items, and have them in the center
    let layout = UICollectionViewFlowLayout()
    layout.itemSize = CGSize(width: itemWidth, height: itemWidth)
    layout.minimumInteritemSpacing = minItemSpacing
    layout.minimumLineSpacing = minItemSpacing
    layout.headerReferenceSize = CGSize(width: 0, height: headerHeight)

    // Find n, where n is the number of item that can fit into the collection view
    var n: CGFloat = 1
    let containerWidth = collectionView.bounds.width
    while true {
        let nextN = n + 1
        let totalWidth = (nextN*itemWidth) + (nextN-1)*minItemSpacing
        if totalWidth > containerWidth {
            break
        } else {
            n = nextN
        }
    }

    // Calculate the section inset for left and right. 
    // Setting this section inset will manipulate the items such that they will all be aligned horizontally center.
    let inset = max(minItemSpacing, floor( (containerWidth - (n*itemWidth) - (n-1)*minItemSpacing) / 2 ) )
    layout.sectionInset = UIEdgeInsets(top: minItemSpacing, left: inset, bottom: minItemSpacing, right: inset)

    collectionView.collectionViewLayout = layout
}

请注意属性名minimumInterItemSpacing。这将是项目之间的最小间距，而不是精确的间距。如果你设置minimumInterItemSpacing为某个值，你可以确保spacing不会小于该值。但有机会获得更高的价值。

实际上，项目之间的间距取决于几个因素itemSize和sectionInset。集合视图根据这些值动态放置内容。所以你不能保证精确的间距。你应该用sectionInset和minimumInterItemSpacing做一些试错。

我有问题与接受的答案，所以我更新了它，这是为我工作:

.h

@interface MaxSpacingCollectionViewFlowLayout : UICollectionViewFlowLayout
@property (nonatomic,assign) CGFloat maxCellSpacing;
@end

.m

@implementation MaxSpacingCollectionViewFlowLayout

- (NSArray *) layoutAttributesForElementsInRect:(CGRect)rect {
    NSArray *attributes = [super layoutAttributesForElementsInRect:rect];

    if (attributes.count <= 0) return attributes;

    CGFloat firstCellOriginX = ((UICollectionViewLayoutAttributes *)attributes[0]).frame.origin.x;

    for(int i = 1; i < attributes.count; i++) {
        UICollectionViewLayoutAttributes *currentLayoutAttributes = attributes[i];
        if (currentLayoutAttributes.frame.origin.x == firstCellOriginX) { // The first cell of a new row
            continue;
        }

        UICollectionViewLayoutAttributes *prevLayoutAttributes = attributes[i - 1];
        CGFloat prevOriginMaxX = CGRectGetMaxX(prevLayoutAttributes.frame);
        if ((currentLayoutAttributes.frame.origin.x - prevOriginMaxX) > self.maxCellSpacing) {
            CGRect frame = currentLayoutAttributes.frame;
            frame.origin.x = prevOriginMaxX + self.maxCellSpacing;
            currentLayoutAttributes.frame = frame;
        }
    }
    return attributes;
}

@end

Swift 5 UIKit编程

 //Create UICollectionView
lazy var collectionView: UICollectionView = {
    
    let layout = UICollectionViewFlowLayout()
    layout.scrollDirection = .horizontal
    
    //CollectionCellView width autoSize
    layout.estimatedItemSize = UICollectionViewFlowLayout.automaticSize
    
    let collectionView = UICollectionView(frame: .zero, collectionViewLayout: layout)
    collectionView.translatesAutoresizingMaskIntoConstraints = false
    
    collectionView.register(CustomCollectionViewCell.self, forCellWithReuseIdentifier: cellId)
    collectionView.backgroundColor = .clear
    collectionView.delegate = self
    collectionView.dataSource = self
    collectionView.alwaysBounceHorizontal = true

//Add Spacing in each cell
    layout.minimumInteritemSpacing = 15
    layout.minimumLineSpacing = 15
    
    return collectionView
}()