试试这个方法:

- (UIEdgeInsets)collectionView:(UICollectionView *)collectionView
                    layout:(UICollectionViewLayout*)collectionViewLayout
    insetForSectionAtIndex:(NSInteger)section {

    UIEdgeInsets insets = UIEdgeInsetsMake(?, ?, ?, ?);

    return insets;
}

Swift 5 UIKit编程

 //Create UICollectionView
lazy var collectionView: UICollectionView = {
    
    let layout = UICollectionViewFlowLayout()
    layout.scrollDirection = .horizontal
    
    //CollectionCellView width autoSize
    layout.estimatedItemSize = UICollectionViewFlowLayout.automaticSize
    
    let collectionView = UICollectionView(frame: .zero, collectionViewLayout: layout)
    collectionView.translatesAutoresizingMaskIntoConstraints = false
    
    collectionView.register(CustomCollectionViewCell.self, forCellWithReuseIdentifier: cellId)
    collectionView.backgroundColor = .clear
    collectionView.delegate = self
    collectionView.dataSource = self
    collectionView.alwaysBounceHorizontal = true

//Add Spacing in each cell
    layout.minimumInteritemSpacing = 15
    layout.minimumLineSpacing = 15
    
    return collectionView
}()

试试下面的代码，确保每个项目之间的间距为5px:

- (UIEdgeInsets)collectionView:(UICollectionView *) collectionView 
                        layout:(UICollectionViewLayout *) collectionViewLayout 
        insetForSectionAtIndex:(NSInteger) section {

    return UIEdgeInsetsMake(0, 0, 0, 5); // top, left, bottom, right
}

- (CGFloat)collectionView:(UICollectionView *) collectionView
                   layout:(UICollectionViewLayout *) collectionViewLayout
  minimumInteritemSpacingForSectionAtIndex:(NSInteger) section {    
    return 5.0;
}

上面vojtech-vrbka的解决方案是正确的，但它会触发一个警告:

警告:UICollectionViewFlowLayout缓存的帧不匹配索引路径-缓存值:这可能是因为流布局子类layout是修改UICollectionViewFlowLayout返回的属性而没有复制它们

下面的代码应该修复它:

class CustomViewFlowLayout : UICollectionViewFlowLayout {

   let cellSpacing:CGFloat = 4

   override func layoutAttributesForElementsInRect(rect: CGRect) -> [UICollectionViewLayoutAttributes]? {
       let original = super.layoutAttributesForElementsInRect(rect)

       if let original = original {
           let attributes = NSArray.init(array: original, copyItems: true) as! [UICollectionViewLayoutAttributes]

           for (index, attribute) in attributes.enumerate() {
                if index == 0 { continue }
                let prevLayoutAttributes = attributes[index - 1]
                let origin = CGRectGetMaxX(prevLayoutAttributes.frame)
                if(origin + cellSpacing + attribute.frame.size.width < self.collectionViewContentSize().width) {
                    attribute.frame.origin.x = origin + cellSpacing
                }
            }
            return attributes
       }
       return nil
   }
}

间距的简单代码

let layout = collectionView.collectionViewLayout as! UICollectionViewFlowLayout
layout.minimumInteritemSpacing = 10 // Some float value

记住，它是最小行间距，而不是最小项间距或单元格间距。因为你的collectionView的滚动方向是水平的。

如果是垂直的，则需要为单元格之间的水平空间设置单元格空间或项间空间，为单元格之间的垂直空间设置行间距。

objective - c版本

- (CGFloat)collectionView:(UICollectionView *)collectionView
                       layout:(UICollectionViewLayout*)collectionViewLayout
    minimumLineSpacingForSectionAtIndex:(NSInteger)section
    {
        return 20;
    }

斯威夫特版本:

func collectionView(_ collectionView: UICollectionView, layout collectionViewLayout: UICollectionViewLayout, minimumLineSpacingForSectionAt section: Int) -> CGFloat {
    return 20
}