查看File对象的.read()方法:

http://docs.python.org/2/tutorial/inputoutput.html#methods-of-file-objects

你可以这样做:

concat = ""
for file in files:
    concat += open(file).read()

或者更“优雅”的python方式:

concat = ''.join([open(f).read() for f in files])

根据这篇文章，http://www.skymind.com/~ocrow/python_string/也将是最快的。

这样就行了

对于大文件:

filenames = ['file1.txt', 'file2.txt', ...]
with open('path/to/output/file', 'w') as outfile:
    for fname in filenames:
        with open(fname) as infile:
            for line in infile:
                outfile.write(line)

对于小文件:

filenames = ['file1.txt', 'file2.txt', ...]
with open('path/to/output/file', 'w') as outfile:
    for fname in filenames:
        with open(fname) as infile:
            outfile.write(infile.read())

我还想到了另一个有趣的问题:

filenames = ['file1.txt', 'file2.txt', ...]
with open('path/to/output/file', 'w') as outfile:
    for line in itertools.chain.from_iterable(itertools.imap(open, filnames)):
        outfile.write(line)

遗憾的是，最后一个方法留下了一些打开的文件描述符，无论如何GC都应该处理这些描述符。我只是觉得很有趣

如果目录中有很多文件，那么glob2可能是生成文件名列表的更好选择，而不是手工编写它们。

import glob2

filenames = glob2.glob('*.txt')  # list of all .txt files in the directory

with open('outfile.txt', 'w') as f:
    for file in filenames:
        with open(file) as infile:
            f.write(infile.read()+'\n')

如果文件不是很大:

with open('newfile.txt','wb') as newf:
    for filename in list_of_files:
        with open(filename,'rb') as hf:
            newf.write(hf.read())
            # newf.write('\n\n\n')   if you want to introduce
            # some blank lines between the contents of the copied files

如果文件太大，不能完全读取并保存在RAM中，则算法必须稍微不同，以固定长度的块读取循环中复制的每个文件，例如使用read(10000)。

@inspectorG4dget答案的替代答案(到2016年3月29日为止的最佳答案)。我测试了3个436MB的文件。

@inspectorG4dget答案:162秒

解决方案:125秒

from subprocess import Popen
filenames = ['file1.txt', 'file2.txt', 'file3.txt']
fbatch = open('batch.bat','w')
str ="type "
for f in filenames:
    str+= f + " "
fbatch.write(str + " > file4results.txt")
fbatch.close()
p = Popen("batch.bat", cwd=r"Drive:\Path\to\folder")
stdout, stderr = p.communicate()

其思想是创建一个批处理文件并执行它，利用“旧的好技术”。它是半python，但运行速度更快。适用于windows。

  import os
  files=os.listdir()
  print(files)
  print('#',tuple(files))
  name=input('Enter the inclusive file name: ')
  exten=input('Enter the type(extension): ')
  filename=name+'.'+exten
  output_file=open(filename,'w+')
  for i in files:
    print(i)
    j=files.index(i)
    f_j=open(i,'r')
    print(f_j.read())
    for x in f_j:
      outfile.write(x)