试试看：

rows = int(input('Enter rows\n'))
my_list = []
for i in range(rows):
    my_list.append(list(map(int, input().split())))

为便于阅读而重写：

# 2D array/ matrix

# 5 rows, 5 cols
rows_count = 5
cols_count = 5

# create
#     creation looks reverse
#     create an array of "cols_count" cols, for each of the "rows_count" rows
#        all elements are initialized to 0
two_d_array = [[0 for j in range(cols_count)] for i in range(rows_count)]

# index is from 0 to 4
#     for both rows & cols
#     since 5 rows, 5 cols

# use
two_d_array[0][0] = 1
print two_d_array[0][0]  # prints 1   # 1st row, 1st col (top-left element of matrix)

two_d_array[1][0] = 2
print two_d_array[1][0]  # prints 2   # 2nd row, 1st col

two_d_array[1][4] = 3
print two_d_array[1][4]  # prints 3   # 2nd row, last col

two_d_array[4][4] = 4
print two_d_array[4][4]  # prints 4   # last row, last col (right, bottom element of matrix)

# Creates a list containing 5 lists initialized to 0
Matrix = [[0]*5]*5

注意这个简短的表达，请参见@F.J的答案中的完整解释

如果您希望能够将其视为2D阵列，而不是被迫根据列表进行思考（在我看来更自然），可以执行以下操作：

import numpy
Nx=3; Ny=4
my2Dlist= numpy.zeros((Nx,Ny)).tolist()

结果是一个列表（不是NumPy数组），您可以用数字、字符串等覆盖各个位置。

l=[[0]*(L) for _ in range(W)]

将快于：

l = [[0 for x in range(L)] for y in range(W)] 

要声明一个零（1）矩阵：

numpy.zeros((x, y))

e.g.

>>> numpy.zeros((3, 5))
    array([[ 0.,  0.,  0.,  0.,  0.],
   [ 0.,  0.,  0.,  0.,  0.],
   [ 0.,  0.,  0.,  0.,  0.]])

或numpy.ones（（x，y））例如

>>> np.ones((3, 5))
array([[ 1.,  1.,  1.,  1.,  1.],
   [ 1.,  1.,  1.,  1.,  1.],
   [ 1.,  1.,  1.,  1.,  1.]])

甚至三维都是可能的。(http://www.astro.ufl.edu/~warner/prog/python.html请参见-->多维数组）