下面是Python 3.5的一行代码：

import string
"l*ots! o(f. p@u)n[c}t]u[a'ti\"on#$^?/".translate(str.maketrans({a:None for a in string.punctuation}))

这里有一个使用RegEx的简单方法

import re

punct = re.compile(r'(\w+)')

sentence = 'This ! is : a # sample $ sentence.' # Text with punctuation
tokenized = [m.group() for m in punct.finditer(sentence)]
sentence = ' '.join(tokenized)
print(sentence) 
'This is a sample sentence'

>>> s = "string. With. Punctuation?"
>>> s = re.sub(r'[^\w\s]','',s)
>>> re.split(r'\s*', s)


['string', 'With', 'Punctuation']

with open('one.txt','r')as myFile:

    str1=myFile.read()

    print(str1)


    punctuation = ['(', ')', '?', ':', ';', ',', '.', '!', '/', '"', "'"] 

for i in punctuation:

        str1 = str1.replace(i," ") 
        myList=[]
        myList.extend(str1.split(" "))
print (str1) 
for i in myList:

    print(i,end='\n')
    print ("____________")

不一定更简单，但如果你更熟悉re家族的话，就另辟蹊径。

import re, string
s = "string. With. Punctuation?" # Sample string 
out = re.sub('[%s]' % re.escape(string.punctuation), '', s)

为什么你们都不用这个？

 ''.join(filter(str.isalnum, s)) 

太慢了？