试试这个:

[[UINavigationBar appearance] setBackgroundImage: [UIImage new]  
                                   forBarMetrics: UIBarMetricsDefault];

[UINavigationBar appearance].shadowImage = [UIImage new];

下图有解释(iOS7导航栏):

检查这个SO问题: iOS7 -改变UINavigationBar的边框颜色

从iOS 13开始，就有一个系统API来设置或删除阴影

UIKit使用shadowImage和shadowColor属性来确定阴影的 外观。当shadowImage为nil时，该工具条显示默认的着色阴影 根据shadowColor属性中的值。如果shadowColor为nil或 包含clearColor颜色，条显示没有阴影。

    let appearance = UINavigationBarAppearance()
    appearance.shadowImage = nil
    appearance.shadowColor = nil
    navigationController.navigationBar.standardAppearance = appearance

https://developer.apple.com/documentation/uikit/uibarappearance/3198009-shadowimage

想把Serhii回答的Swift版本加进去。我用下面的代码创建了一个uibareextension .swift:

import Foundation
import UIKit

extension UINavigationBar {
    func hideBottomHairline() {
        self.hairlineImageView?.isHidden = true
    }

    func showBottomHairline() {
        self.hairlineImageView?.isHidden = false
    }
}

extension UIToolbar {
    func hideBottomHairline() {
        self.hairlineImageView?.isHidden = true
    }

    func showBottomHairline() {
        self.hairlineImageView?.isHidden = false
    }
}

extension UIView {
    fileprivate var hairlineImageView: UIImageView? {
        return hairlineImageView(in: self)
    }

    fileprivate func hairlineImageView(in view: UIView) -> UIImageView? {
        if let imageView = view as? UIImageView, imageView.bounds.height <= 1.0 {
            return imageView
        }

        for subview in view.subviews {
            if let imageView = self.hairlineImageView(in: subview) { return imageView }
        }

        return nil
    }
}

        if #available(iOS 13.0, *) {
            let appearance = UINavigationBarAppearance()
            appearance.backgroundColor          = Colors.color_app
            appearance.titleTextAttributes      = [.foregroundColor : UIColor.white]
            appearance.largeTitleTextAttributes = [.foregroundColor : UIColor.white]
            appearance.shadowColor = .clear
            appearance.shadowImage = UIImage()
            
            UINavigationBar.appearance().tintColor            = .white
            UINavigationBar.appearance().standardAppearance   = appearance
            UINavigationBar.appearance().compactAppearance    = appearance
            UINavigationBar.appearance().scrollEdgeAppearance = appearance
        } else {
            UINavigationBar.appearance().barTintColor        = Colors.color_app
            UINavigationBar.appearance().tintColor           = .white
            UINavigationBar.appearance().titleTextAttributes = [NSAttributedString.Key.foregroundColor : UIColor.white]
            if #available(iOS 11.0, *) {
                UINavigationBar.appearance().largeTitleTextAttributes = [NSAttributedString.Key.foregroundColor: UIColor.white]
            }
            UINavigationBar.appearance().isTranslucent = false
            
            UINavigationBar.appearance().shadowImage = UIImage()
            UINavigationBar.appearance().setBackgroundImage(UIImage(), for: .default)
        }

pxpgraphics的解决方案为Swift 2.0更新

extension UINavigationBar {

    func hideBottomHairline()
    {
        hairlineImageViewInNavigationBar(self)?.hidden = true
    }

    func showBottomHairline()
    {
        hairlineImageViewInNavigationBar(self)?.hidden = false
    }

    private func hairlineImageViewInNavigationBar(view: UIView) -> UIImageView?
    {
        if let imageView = view as? UIImageView where imageView.bounds.height <= 1
        {
            return imageView
        }

        for subview: UIView in view.subviews
        {
            if let imageView = hairlineImageViewInNavigationBar(subview)
            {
                return imageView
            }
        }

        return nil
    }

}

extension UIToolbar
{

    func hideHairline()
    {
        let navigationBarImageView = hairlineImageViewInToolbar(self)?.hidden = true
    }

    func showHairline()
    {
        let navigationBarImageView = hairlineImageViewInToolbar(self)?.hidden = false
    }

    private func hairlineImageViewInToolbar(view: UIView) -> UIImageView?
    {
        if let imageView = view as? UIImageView where imageView.bounds.height <= 1
        {
            return imageView
        }

        for subview: UIView in view.subviews
        {
            if let imageView = hairlineImageViewInToolbar(subview)
            {
                return imageView
            }
        }

        return nil
    }

}

[tabviewController.view setBackgroundColor:[UIColor blackColor]];

对我来说[UIColor blackColor]可能是你的背景色， tabviewController是你的UITabBarController，如果你正在使用它!