对于iOS 9用户，这对我来说很有效。只要加上这个:

UINavigationBar.appearance().shadowImage = UIImage()

我使用UINavigationBar扩展，使我能够隐藏/显示使用UIAppearance API的阴影或选择哪个导航栏必须隐藏/显示使用故事板(或源代码)的阴影。这是扩展:

import UIKit

private var flatAssociatedObjectKey: UInt8 = 0

/*
  An extension that adds a "flat" field to UINavigationBar. This flag, when
  enabled, removes the shadow under the navigation bar.
 */
@IBDesignable extension UINavigationBar {
    @IBInspectable var flat: Bool {
        get {
            guard let obj = objc_getAssociatedObject(self, &flatAssociatedObjectKey) as? NSNumber else {
                return false
            }
            return obj.boolValue;
        }

        set {
            if (newValue) {
                let void = UIImage()
                setBackgroundImage(void, forBarPosition: .Any, barMetrics: .Default)
                shadowImage = void
            } else {
                setBackgroundImage(nil, forBarPosition: .Any, barMetrics: .Default)
                shadowImage = nil
            }
            objc_setAssociatedObject(self, &flatAssociatedObjectKey, NSNumber(bool: newValue),
                    objc_AssociationPolicy.OBJC_ASSOCIATION_RETAIN_NONATOMIC)
        }
    }
}

现在，要禁用所有导航条上的阴影，你必须使用:

UINavigationBar.appearance().flat = true

或者你可以使用故事板启用/禁用此行为:

简单的解决方案

   let navigationBar = self.navigationController?.navigationBar
    navigationBar?.setBackgroundImage(UIImage(), forBarPosition: UIBarPosition.Any, barMetrics: UIBarMetrics.Default)
    navigationBar?.shadowImage = UIImage()

斯威夫特4 //隐藏导航栏阴影线

navigationController?.navigationBar.shadowImage = UIImage()

Slightly Swift Solution 
func setGlobalAppearanceCharacteristics () {
    let navigationBarAppearace = UINavigationBar.appearance()
    navigationBarAppearace.tintColor = UIColor.white
    navigationBarAppearace.barTintColor = UIColor.blue
    navigationBarAppearace.setBackgroundImage(UIImage(), for: UIBarMetrics.default)
    navigationBarAppearace.shadowImage = UIImage()

}

我也遇到了同样的问题，但没有一个答案真正令人满意。以下是我对Swift3的看法:

func hideNavigationBarLine() {
    navigationController?.navigationBar.setBackgroundImage(UIImage(), for: .default)
    navigationController?.navigationBar.shadowImage = UIImage()
}

只需在viewDidLoad()中调用它。