@OP，你可以使用枚举

for n,line in enumerate(open("file")):
    if n+1 in [26,30]: # or n in [25,29] 
       print line.rstrip()

这是我的2美分，不管它是否值得;)

def indexLines(filename, lines=[2,4,6,8,10,12,3,5,7,1]):
    fp   = open(filename, "r")
    src  = fp.readlines()
    data = [(index, line) for index, line in enumerate(src) if index in lines]
    fp.close()
    return data


# Usage below
filename = "C:\\Your\\Path\\And\\Filename.txt"
for line in indexLines(filename): # using default list, specify your own list of lines otherwise
    print "Line: %s\nData: %s\n" % (line[0], line[1])

with open("test.txt", "r") as fp:
   lines = fp.readlines()
print(lines[3])

Test.txt是文件名 打印test.txt中的第4行

为了完整起见，这里还有一个选项。

让我们从python文档中的定义开始:

通常包含序列的一部分的对象。slice使用下标符号[]创建，当给出几个数字时，数字之间使用冒号，例如variable_name[1:3:5]。括号(下标)表示法在内部使用切片对象(或在旧版本中使用__getslice__()和__setslice__())。

虽然slice表示法一般不直接适用于迭代器，但itertools包包含一个替换函数:

from itertools import islice

# print the 100th line
with open('the_file') as lines:
    for line in islice(lines, 99, 100):
        print line

# print each third line until 100
with open('the_file') as lines:
    for line in islice(lines, 0, 100, 3):
        print line

该函数的另一个优点是，它直到结束才读取迭代器。所以你可以做更复杂的事情:

with open('the_file') as lines:
    # print the first 100 lines
    for line in islice(lines, 100):
        print line

    # then skip the next 5
    for line in islice(lines, 5):
        pass

    # print the rest
    for line in lines:
        print line

为了回答最初的问题:

# how to read lines #26 and #30
In [365]: list(islice(xrange(1,100), 25, 30, 4))
Out[365]: [26, 30]

如果你不介意导入，那么fileinput确实是你需要的(这是你可以读取当前行的行号)

我认为这行得通

 open_file1 = open("E:\\test.txt",'r')
 read_it1 = open_file1.read()
 myline1 = []
 for line1 in read_it1.splitlines():
 myline1.append(line1)
 print myline1[0]