我认为这行得通

 open_file1 = open("E:\\test.txt",'r')
 read_it1 = open_file1.read()
 myline1 = []
 for line1 in read_it1.splitlines():
 myline1.append(line1)
 print myline1[0]

我更喜欢这种方法，因为它更通用，即你可以在文件上使用它，在f.r edlines()的结果上，在StringIO对象上，无论什么:

def read_specific_lines(file, lines_to_read):
   """file is any iterable; lines_to_read is an iterable containing int values"""
   lines = set(lines_to_read)
   last = max(lines)
   for n, line in enumerate(file):
      if n + 1 in lines:
          yield line
      if n + 1 > last:
          return

>>> with open(r'c:\temp\words.txt') as f:
        [s for s in read_specific_lines(f, [1, 2, 3, 1000])]
['A\n', 'a\n', 'aa\n', 'accordant\n']

def getitems(iterable, items):
  items = list(items) # get a list from any iterable and make our own copy
                      # since we modify it
  if items:
    items.sort()
    for n, v in enumerate(iterable):
      if n == items[0]:
        yield v
        items.pop(0)
        if not items:
          break

print list(getitems(open("/usr/share/dict/words"), [25, 29]))
# ['Abelson\n', 'Abernathy\n']
# note that index 25 is the 26th item

文件对象有一个.readlines()方法，它将为您提供文件内容的列表，每个列表项一行。在此之后，您可以使用普通的列表切片技术。

http://docs.python.org/library/stdtypes.html#file.readlines

如果你不介意导入，那么fileinput确实是你需要的(这是你可以读取当前行的行号)

@OP，你可以使用枚举

for n,line in enumerate(open("file")):
    if n+1 in [26,30]: # or n in [25,29] 
       print line.rstrip()