下面是如何使用PIL (install with: pip install Pillow):

import glob
import contextlib
from PIL import Image

# filepaths
fp_in = "/path/to/image_*.png"
fp_out = "/path/to/image.gif"

# use exit stack to automatically close opened images
with contextlib.ExitStack() as stack:

    # lazily load images
    imgs = (stack.enter_context(Image.open(f))
            for f in sorted(glob.glob(fp_in)))

    # extract  first image from iterator
    img = next(imgs)

    # https://pillow.readthedocs.io/en/stable/handbook/image-file-formats.html#gif
    img.save(fp=fp_out, format='GIF', append_images=imgs,
             save_all=True, duration=200, loop=0)

查看文档:https://pillow.readthedocs.io/en/stable/handbook/image-file-formats.html#gif

现在我在用ImageMagick。我将帧保存为PNG文件，然后从Python中调用ImageMagick的convert.exe来创建一个动画GIF。这种方法的好处是我可以为每一帧单独指定帧持续时间。不幸的是，这取决于ImageMagick安装在机器上。他们有一个Python包装器，但它看起来很糟糕，不受支持。还在考虑其他建议。

它不是一个python库，但mencoder可以做到这一点:从多个输入图像文件编码。你可以像这样从python中执行mencoder:

import os

os.system("mencoder ...")

from PIL import Image
import glob  #use it if you want to read all of the certain file type in the directory
imgs=[]
for i in range(596,691): 
    imgs.append("snap"+str(i)+'.png')
    print("scanned the image identified with",i)  

标识不同文件名的索引的起始值和结束值+1

imgs = glob.glob("*.png") #do this if you want to read all files ending with .png

我的文件是:snap596.png, snap597.png ......snap690.png

frames = []
for i in imgs:
    new_frame = Image.open(i)
    frames.append(new_frame)

保存到一个GIF文件，永远循环

frames[0].save('fire3_PIL.gif', format='GIF',
    append_images=frames[1:],
    save_all=True,
    duration=300, loop=0)

我发现闪烁的问题与imageio和这个方法固定。

我偶然发现了PIL的ImageSequence模块，它提供了更好(更标准)的GIF动画。这次我也使用了Tk的after()方法，这比time.sleep()更好。

from Tkinter import * 
from PIL import Image, ImageTk, ImageSequence

def stop(event):
  global play
  play = False
  exit() 

root = Tk()
root.bind("<Key>", stop) # Press any key to stop
GIFfile = {path_to_your_GIF_file}
im = Image.open(GIFfile); img = ImageTk.PhotoImage(im)
delay = im.info['duration'] # Delay used in the GIF file 
lbl = Label(image=img); lbl.pack() # Create a label where to display images
play = True;
while play:
  for frame in ImageSequence.Iterator(im):
    if not play: break 
    root.after(delay);
    img = ImageTk.PhotoImage(frame)
    lbl.config(image=img); root.update() # Show the new frame/image

root.mainloop()

老问题，有很多很好的答案，但可能仍然对另一种选择感兴趣……

我最近在github (https://github.com/WarrenWeckesser/numpngw)上发布的numpngw模块可以从numpy数组写入动画PNG文件。(更新:numpngw现在在pypi: https://pypi.python.org/pypi/numpngw。)

例如，这个脚本:

import numpy as np
import numpngw


img0 = np.zeros((64, 64, 3), dtype=np.uint8)
img0[:32, :32, :] = 255
img1 = np.zeros((64, 64, 3), dtype=np.uint8)
img1[32:, :32, 0] = 255
img2 = np.zeros((64, 64, 3), dtype=np.uint8)
img2[32:, 32:, 1] = 255
img3 = np.zeros((64, 64, 3), dtype=np.uint8)
img3[:32, 32:, 2] = 255
seq = [img0, img1, img2, img3]
for img in seq:
    img[16:-16, 16:-16] = 127
    img[0, :] = 127
    img[-1, :] = 127
    img[:, 0] = 127
    img[:, -1] = 127

numpngw.write_apng('foo.png', seq, delay=250, use_palette=True)

创建:

你需要一个支持动画PNG的浏览器(直接或带有插件)才能看到动画。