使用已接受的答案，我创建了一个帮助类，以便您可以以类似于使用Lock语句的方式使用它。只是想分享一下。

使用:

using (new SingleGlobalInstance(1000)) //1000ms timeout on global lock
{
    //Only 1 of these runs at a time
    RunSomeStuff();
}

helper类:

class SingleGlobalInstance : IDisposable
{
    //edit by user "jitbit" - renamed private fields to "_"
    public bool _hasHandle = false;
    Mutex _mutex;

    private void InitMutex()
    {
        string appGuid = ((GuidAttribute)Assembly.GetExecutingAssembly().GetCustomAttributes(typeof(GuidAttribute), false).GetValue(0)).Value;
        string mutexId = string.Format("Global\\{{{0}}}", appGuid);
        _mutex = new Mutex(false, mutexId);

        var allowEveryoneRule = new MutexAccessRule(new SecurityIdentifier(WellKnownSidType.WorldSid, null), MutexRights.FullControl, AccessControlType.Allow);
        var securitySettings = new MutexSecurity();
        securitySettings.AddAccessRule(allowEveryoneRule);
        _mutex.SetAccessControl(securitySettings);
    }

    public SingleGlobalInstance(int timeOut)
    {
        InitMutex();
        try
        {
            if(timeOut < 0)
                _hasHandle = _mutex.WaitOne(Timeout.Infinite, false);
            else
                _hasHandle = _mutex.WaitOne(timeOut, false);

            if (_hasHandle == false)
                throw new TimeoutException("Timeout waiting for exclusive access on SingleInstance");
        }
        catch (AbandonedMutexException)
        {
            _hasHandle = true;
        }
    }


    public void Dispose()
    {
        if (_mutex != null)
        {
            if (_hasHandle)
                _mutex.ReleaseMutex();
            _mutex.Close();
        }
    }
}

我想要确保这是存在的，因为它很难做到正确:

using System.Runtime.InteropServices;   //GuidAttribute
using System.Reflection;                //Assembly
using System.Threading;                 //Mutex
using System.Security.AccessControl;    //MutexAccessRule
using System.Security.Principal;        //SecurityIdentifier

static void Main(string[] args)
{
    // get application GUID as defined in AssemblyInfo.cs
    string appGuid =
        ((GuidAttribute)Assembly.GetExecutingAssembly().
            GetCustomAttributes(typeof(GuidAttribute), false).
                GetValue(0)).Value.ToString();

    // unique id for global mutex - Global prefix means it is global to the machine
    string mutexId = string.Format( "Global\\{{{0}}}", appGuid );

    // Need a place to store a return value in Mutex() constructor call
    bool createdNew;

    // edited by Jeremy Wiebe to add example of setting up security for multi-user usage
    // edited by 'Marc' to work also on localized systems (don't use just "Everyone") 
    var allowEveryoneRule =
        new MutexAccessRule( new SecurityIdentifier( WellKnownSidType.WorldSid
                                                   , null)
                           , MutexRights.FullControl
                           , AccessControlType.Allow
                           );
    var securitySettings = new MutexSecurity();
    securitySettings.AddAccessRule(allowEveryoneRule);

   // edited by MasonGZhwiti to prevent race condition on security settings via VanNguyen
    using (var mutex = new Mutex(false, mutexId, out createdNew, securitySettings))
    {
        // edited by acidzombie24
        var hasHandle = false;
        try
        {
            try
            {
                // note, you may want to time out here instead of waiting forever
                // edited by acidzombie24
                // mutex.WaitOne(Timeout.Infinite, false);
                hasHandle = mutex.WaitOne(5000, false);
                if (hasHandle == false)
                    throw new TimeoutException("Timeout waiting for exclusive access");
            }
            catch (AbandonedMutexException)
            {
                // Log the fact that the mutex was abandoned in another process,
                // it will still get acquired
                hasHandle = true;
            }

            // Perform your work here.
        }
        finally
        {
            // edited by acidzombie24, added if statement
            if(hasHandle)
                mutex.ReleaseMutex();
        }
    }
}

Mutex和WinApi CreateMutex()都不适合我。

另一种解决方案:

static class Program
{
    [STAThread]
    static void Main()
    {
        if (SingleApplicationDetector.IsRunning()) {
            return;
        }

        Application.Run(new MainForm());

        SingleApplicationDetector.Close();
    }
}

和SingleApplicationDetector:

using System;
using System.Reflection;
using System.Runtime.InteropServices;
using System.Security.AccessControl;
using System.Threading;

public static class SingleApplicationDetector
{
    public static bool IsRunning()
    {
        string guid = ((GuidAttribute)Assembly.GetExecutingAssembly().GetCustomAttributes(typeof(GuidAttribute), false).GetValue(0)).Value.ToString();
        var semaphoreName = @"Global\" + guid;
        try {
            __semaphore = Semaphore.OpenExisting(semaphoreName, SemaphoreRights.Synchronize);

            Close();
            return true;
        }
        catch (Exception ex) {
            __semaphore = new Semaphore(0, 1, semaphoreName);
            return false;
        }
    }

    public static void Close()
    {
        if (__semaphore != null) {
            __semaphore.Close();
            __semaphore = null;
        }
    }

    private static Semaphore __semaphore;
}

使用信号量而不是互斥锁的原因:

Mutex类强制线程标识，因此只有获得互斥锁的线程才能释放它。相反，Semaphore类并不强制执行线程标识。 < < System.Threading.Mutex

裁判:Semaphore.OpenExisting ()

使用已接受的答案，我创建了一个帮助类，以便您可以以类似于使用Lock语句的方式使用它。只是想分享一下。

使用:

using (new SingleGlobalInstance(1000)) //1000ms timeout on global lock
{
    //Only 1 of these runs at a time
    RunSomeStuff();
}

helper类:

class SingleGlobalInstance : IDisposable
{
    //edit by user "jitbit" - renamed private fields to "_"
    public bool _hasHandle = false;
    Mutex _mutex;

    private void InitMutex()
    {
        string appGuid = ((GuidAttribute)Assembly.GetExecutingAssembly().GetCustomAttributes(typeof(GuidAttribute), false).GetValue(0)).Value;
        string mutexId = string.Format("Global\\{{{0}}}", appGuid);
        _mutex = new Mutex(false, mutexId);

        var allowEveryoneRule = new MutexAccessRule(new SecurityIdentifier(WellKnownSidType.WorldSid, null), MutexRights.FullControl, AccessControlType.Allow);
        var securitySettings = new MutexSecurity();
        securitySettings.AddAccessRule(allowEveryoneRule);
        _mutex.SetAccessControl(securitySettings);
    }

    public SingleGlobalInstance(int timeOut)
    {
        InitMutex();
        try
        {
            if(timeOut < 0)
                _hasHandle = _mutex.WaitOne(Timeout.Infinite, false);
            else
                _hasHandle = _mutex.WaitOne(timeOut, false);

            if (_hasHandle == false)
                throw new TimeoutException("Timeout waiting for exclusive access on SingleInstance");
        }
        catch (AbandonedMutexException)
        {
            _hasHandle = true;
        }
    }


    public void Dispose()
    {
        if (_mutex != null)
        {
            if (_hasHandle)
                _mutex.ReleaseMutex();
            _mutex.Close();
        }
    }
}

如果另一个实例已经在运行，则此示例将在5秒后退出。

// unique id for global mutex - Global prefix means it is global to the machine
const string mutex_id = "Global\\{B1E7934A-F688-417f-8FCB-65C3985E9E27}";

static void Main(string[] args)
{

    using (var mutex = new Mutex(false, mutex_id))
    {
        try
        {
            try
            {
                if (!mutex.WaitOne(TimeSpan.FromSeconds(5), false))
                {
                    Console.WriteLine("Another instance of this program is running");
                    Environment.Exit(0);
                }
            }
            catch (AbandonedMutexException)
            {
                // Log the fact the mutex was abandoned in another process, it will still get aquired
            }

            // Perform your work here.
        }
        finally
        {
            mutex.ReleaseMutex();
        }
    }
}

Sometimes learning by example helps the most. Run this console application in three different console windows. You'll see that the application you ran first acquires the mutex first, while the other two are waiting their turn. Then press enter in the first application, you'll see that application 2 now continues running by acquiring the mutex, however application 3 is waiting its turn. After you press enter in application 2 you'll see that application 3 continues. This illustrates the concept of a mutex protecting a section of code to be executed only by one thread (in this case a process) like writing to a file as an example.

using System;
using System.Threading;

namespace MutexExample
{
    class Program
    {
        static Mutex m = new Mutex(false, "myMutex");//create a new NAMED mutex, DO NOT OWN IT
        static void Main(string[] args)
        {
            Console.WriteLine("Waiting to acquire Mutex");
            m.WaitOne(); //ask to own the mutex, you'll be queued until it is released
            Console.WriteLine("Mutex acquired.\nPress enter to release Mutex");
            Console.ReadLine();
            m.ReleaseMutex();//release the mutex so other processes can use it
        }
    }
}