基于耐克的答案。使用opencv实现基于拉普拉斯的方法很简单:

short GetSharpness(char* data, unsigned int width, unsigned int height)
{
    // assumes that your image is already in planner yuv or 8 bit greyscale
    IplImage* in = cvCreateImage(cvSize(width,height),IPL_DEPTH_8U,1);
    IplImage* out = cvCreateImage(cvSize(width,height),IPL_DEPTH_16S,1);
    memcpy(in->imageData,data,width*height);

    // aperture size of 1 corresponds to the correct matrix
    cvLaplace(in, out, 1);

    short maxLap = -32767;
    short* imgData = (short*)out->imageData;
    for(int i =0;i<(out->imageSize/2);i++)
    {
        if(imgData[i] > maxLap) maxLap = imgData[i];
    }

    cvReleaseImage(&in);
    cvReleaseImage(&out);
    return maxLap;
}

将返回一个短消息，指示检测到的最大锐度，根据我对真实世界样本的测试，这是一个很好的指标，说明相机是否对焦。不足为奇的是，正常值是依赖于场景的，但远不如FFT方法，它有很高的假阳性率，在我的应用程序中是有用的。

基于耐克的答案。使用opencv实现基于拉普拉斯的方法很简单:

short GetSharpness(char* data, unsigned int width, unsigned int height)
{
    // assumes that your image is already in planner yuv or 8 bit greyscale
    IplImage* in = cvCreateImage(cvSize(width,height),IPL_DEPTH_8U,1);
    IplImage* out = cvCreateImage(cvSize(width,height),IPL_DEPTH_16S,1);
    memcpy(in->imageData,data,width*height);

    // aperture size of 1 corresponds to the correct matrix
    cvLaplace(in, out, 1);

    short maxLap = -32767;
    short* imgData = (short*)out->imageData;
    for(int i =0;i<(out->imageSize/2);i++)
    {
        if(imgData[i] > maxLap) maxLap = imgData[i];
    }

    cvReleaseImage(&in);
    cvReleaseImage(&out);
    return maxLap;
}

将返回一个短消息，指示检测到的最大锐度，根据我对真实世界样本的测试，这是一个很好的指标，说明相机是否对焦。不足为奇的是，正常值是依赖于场景的，但远不如FFT方法，它有很高的假阳性率，在我的应用程序中是有用的。

这就是我在Opencv中检测区域焦点质量的方法:

Mat grad;
int scale = 1;
int delta = 0;
int ddepth = CV_8U;
Mat grad_x, grad_y;
Mat abs_grad_x, abs_grad_y;
/// Gradient X
Sobel(matFromSensor, grad_x, ddepth, 1, 0, 3, scale, delta, BORDER_DEFAULT);
/// Gradient Y
Sobel(matFromSensor, grad_y, ddepth, 0, 1, 3, scale, delta, BORDER_DEFAULT);
convertScaleAbs(grad_x, abs_grad_x);
convertScaleAbs(grad_y, abs_grad_y);
addWeighted(abs_grad_x, 0.5, abs_grad_y, 0.5, 0, grad);
cv::Scalar mu, sigma;
cv::meanStdDev(grad, /* mean */ mu, /*stdev*/ sigma);
focusMeasure = mu.val[0] * mu.val[0];

上面的回答阐明了许多事情，但我认为做一个概念上的区分是有用的。

如果你对一个模糊的图像拍摄一张完美对焦的照片呢?

The blurring detection problem is only well posed when you have a reference. If you need to design, e.g., an auto-focus system, you compare a sequence of images taken with different degrees of blurring, or smoothing, and you try to find the point of minimum blurring within this set. I other words you need to cross reference the various images using one of the techniques illustrated above (basically--with various possible levels of refinement in the approach--looking for the one image with the highest high-frequency content).

我想到了一个完全不同的解决方案。 我需要分析视频静止帧，以便在每(X)帧中找到最清晰的帧。这样，我将检测运动模糊和/或失焦图像。

我最终使用了Canny边缘检测，我得到了非常非常好的结果，几乎每一种视频(与尼基的方法，我有数字化的VHS视频和沉重的交错视频的问题)。

我通过在原始图像上设置感兴趣区域(ROI)来优化性能。

使用EmguCV:

//Convert image using Canny
using (Image<Gray, byte> imgCanny = imgOrig.Canny(225, 175))
{
    //Count the number of pixel representing an edge
    int nCountCanny = imgCanny.CountNonzero()[0];

    //Compute a sharpness grade:
    //< 1.5 = blurred, in movement
    //de 1.5 à 6 = acceptable
    //> 6 =stable, sharp
    double dSharpness = (nCountCanny * 1000.0 / (imgCanny.Cols * imgCanny.Rows));
}

我在matlab中使用FFT实现了它，并检查FFT的直方图，计算平均值和STD，还可以做拟合函数

fa =  abs(fftshift(fft(sharp_img)));
fb = abs(fftshift(fft(blured_img)));

f1=20*log10(0.001+fa);
f2=20*log10(0.001+fb);

figure,imagesc(f1);title('org')
figure,imagesc(f2);title('blur')

figure,hist(f1(:),100);title('org')
figure,hist(f2(:),100);title('blur')

mf1=mean(f1(:));
mf2=mean(f2(:));

mfd1=median(f1(:));
mfd2=median(f2(:));

sf1=std(f1(:));
sf2=std(f2(:));