虽然上面的答案是正确的，但对于那些希望在IISNODE中工作的人来说，还需要指定

<configuration>
    <system.webServer>
        <httpErrors existingResponse="PassThrough"/>
    </system.webServer>
<configuration>

在你的网里。配置(否则IIS将吃掉您的输出)。

上面的答案很好，但其中一半的答案不会返回404作为HTTP状态代码，而另一半答案则不能呈现自定义模板。在Expressjs中拥有自定义错误页面(404)的最佳方法是

app.use(function(req, res, next){
    res.status(404).render('404_error_template', {title: "Sorry, page not found"});
});

将此代码放在所有URL映射的末尾。

这种方法更简单，但它必须是所有路线之后的最后一件事。

app.use( (req, res) => {
    //render page not found 
    res.render('404')
})

我发现这个例子很有帮助:

https://github.com/visionmedia/express/blob/master/examples/error-pages/index.js

所以，它实际上是这一部分:

// "app.router" positions our routes
// above the middleware defined below,
// this means that Express will attempt
// to match & call routes _before_ continuing
// on, at which point we assume it's a 404 because
// no route has handled the request.

app.use(app.router);

// Since this is the last non-error-handling
// middleware use()d, we assume 404, as nothing else
// responded.

// $ curl http://localhost:3000/notfound
// $ curl http://localhost:3000/notfound -H "Accept: application/json"
// $ curl http://localhost:3000/notfound -H "Accept: text/plain"

app.use(function(req, res, next) {
  res.status(404);

  // respond with html page
  if (req.accepts('html')) {
    res.render('404', { url: req.url });
    return;
  }

  // respond with json
  if (req.accepts('json')) {
    res.json({ error: 'Not found' });
    return;
  }

  // default to plain-text. send()
  res.type('txt').send('Not found');
});

我在定义所有路由后所做的是捕捉潜在的404并转发到错误处理程序，如下所示:

    const httpError = require('http-errors');

    ...

    // API router
    app.use('/api/', routes);
    
    // catch 404 and forward to error handler
    app.use((req, res, next) => {
      const err = new httpError(404)
      return next(err);
    });

    module.exports = app;

我认为你应该首先定义你所有的路线，然后作为最后的路线添加

//The 404 Route (ALWAYS Keep this as the last route)
app.get('*', function(req, res){
  res.status(404).send('what???');
});

一个示例应用程序的工作:

app.js:

var express = require('express'),
    app = express.createServer();

app.use(express.static(__dirname + '/public'));

app.get('/', function(req, res){
  res.send('hello world');
});

//The 404 Route (ALWAYS Keep this as the last route)
app.get('*', function(req, res){
  res.send('what???', 404);
});

app.listen(3000, '127.0.0.1');

alfred@alfred-laptop:~/node/stackoverflow/6528876$ mkdir public
alfred@alfred-laptop:~/node/stackoverflow/6528876$ find .
alfred@alfred-laptop:~/node/stackoverflow/6528876$ echo "I don't find a function for that... Anyone knows?" > public/README.txt
alfred@alfred-laptop:~/node/stackoverflow/6528876$ cat public/README.txt 

.
./app.js
./public
./public/README.txt

alfred@alfred-laptop:~/node/stackoverflow/6528876$ curl http://localhost:3000/
hello world
alfred@alfred-laptop:~/node/stackoverflow/6528876$ curl http://localhost:3000/README.txt
I don't find a function for that... Anyone knows?