上面接受的答案遇到了一个问题，如果文件已被打开以使用FileShare写入。读取模式或如果文件具有只读属性，代码将无法工作。这个修改后的解决方案最可靠，需要记住两件事(对于公认的解决方案也是如此):

它将不适用于已以写共享模式打开的文件 这没有考虑到线程问题，所以你需要锁定它或单独处理线程问题。

记住上面的内容，这将检查文件是被写入锁定还是被阻止读取锁定:

public static bool FileLocked(string FileName)
{
    FileStream fs = null;

    try
    {
        // NOTE: This doesn't handle situations where file is opened for writing by another process but put into write shared mode, it will not throw an exception and won't show it as write locked
        fs = File.Open(FileName, FileMode.Open, FileAccess.ReadWrite, FileShare.None); // If we can't open file for reading and writing then it's locked by another process for writing
    }
    catch (UnauthorizedAccessException) // https://msdn.microsoft.com/en-us/library/y973b725(v=vs.110).aspx
    {
        // This is because the file is Read-Only and we tried to open in ReadWrite mode, now try to open in Read only mode
        try
        {
            fs = File.Open(FileName, FileMode.Open, FileAccess.Read, FileShare.None);
        }
        catch (Exception)
        {
            return true; // This file has been locked, we can't even open it to read
        }
    }
    catch (Exception)
    {
        return true; // This file has been locked
    }
    finally
    {
        if (fs != null)
            fs.Close();
    }
    return false;
}

使用此命令检查文件是否被锁定:

using System.IO;
using System.Runtime.InteropServices;
internal static class Helper
{
const int ERROR_SHARING_VIOLATION = 32;
const int ERROR_LOCK_VIOLATION = 33;

private static bool IsFileLocked(Exception exception)
{
    int errorCode = Marshal.GetHRForException(exception) & ((1 << 16) - 1);
    return errorCode == ERROR_SHARING_VIOLATION || errorCode == ERROR_LOCK_VIOLATION;
}

internal static bool CanReadFile(string filePath)
{
    //Try-Catch so we dont crash the program and can check the exception
    try {
        //The "using" is important because FileStream implements IDisposable and
        //"using" will avoid a heap exhaustion situation when too many handles  
        //are left undisposed.
        using (FileStream fileStream = File.Open(filePath, FileMode.Open, FileAccess.ReadWrite, FileShare.None)) {
            if (fileStream != null) fileStream.Close();  //This line is me being overly cautious, fileStream will never be null unless an exception occurs... and I know the "using" does it but its helpful to be explicit - especially when we encounter errors - at least for me anyway!
        }
    }
    catch (IOException ex) {
        //THE FUNKY MAGIC - TO SEE IF THIS FILE REALLY IS LOCKED!!!
        if (IsFileLocked(ex)) {
            // do something, eg File.Copy or present the user with a MsgBox - I do not recommend Killing the process that is locking the file
            return false;
        }
    }
    finally
    { }
    return true;
}
}

出于性能考虑，我建议您在同一操作中读取文件内容。下面是一些例子:

public static byte[] ReadFileBytes(string filePath)
{
    byte[] buffer = null;
    try
    {
        using (FileStream fileStream = File.Open(filePath, FileMode.Open, FileAccess.ReadWrite, FileShare.None))
        {
            int length = (int)fileStream.Length;  // get file length
            buffer = new byte[length];            // create buffer
            int count;                            // actual number of bytes read
            int sum = 0;                          // total number of bytes read

            // read until Read method returns 0 (end of the stream has been reached)
            while ((count = fileStream.Read(buffer, sum, length - sum)) > 0)
                sum += count;  // sum is a buffer offset for next reading

            fileStream.Close(); //This is not needed, just me being paranoid and explicitly releasing resources ASAP
        }
    }
    catch (IOException ex)
    {
        //THE FUNKY MAGIC - TO SEE IF THIS FILE REALLY IS LOCKED!!!
        if (IsFileLocked(ex))
        {
            // do something? 
        }
    }
    catch (Exception ex)
    {
    }
    finally
    {
    }
    return buffer;
}

public static string ReadFileTextWithEncoding(string filePath)
{
    string fileContents = string.Empty;
    byte[] buffer;
    try
    {
        using (FileStream fileStream = File.Open(filePath, FileMode.Open, FileAccess.ReadWrite, FileShare.None))
        {
            int length = (int)fileStream.Length;  // get file length
            buffer = new byte[length];            // create buffer
            int count;                            // actual number of bytes read
            int sum = 0;                          // total number of bytes read

            // read until Read method returns 0 (end of the stream has been reached)
            while ((count = fileStream.Read(buffer, sum, length - sum)) > 0)
            {
                sum += count;  // sum is a buffer offset for next reading
            }

            fileStream.Close(); //Again - this is not needed, just me being paranoid and explicitly releasing resources ASAP

            //Depending on the encoding you wish to use - I'll leave that up to you
            fileContents = System.Text.Encoding.Default.GetString(buffer);
        }
    }
    catch (IOException ex)
    {
        //THE FUNKY MAGIC - TO SEE IF THIS FILE REALLY IS LOCKED!!!
        if (IsFileLocked(ex))
        {
            // do something? 
        }
    }
    catch (Exception ex)
    {
    }
    finally
    { }     
    return fileContents;
}

public static string ReadFileTextNoEncoding(string filePath)
{
    string fileContents = string.Empty;
    byte[] buffer;
    try
    {
        using (FileStream fileStream = File.Open(filePath, FileMode.Open, FileAccess.ReadWrite, FileShare.None))
        {
            int length = (int)fileStream.Length;  // get file length
            buffer = new byte[length];            // create buffer
            int count;                            // actual number of bytes read
            int sum = 0;                          // total number of bytes read

            // read until Read method returns 0 (end of the stream has been reached)
            while ((count = fileStream.Read(buffer, sum, length - sum)) > 0) 
            {
                sum += count;  // sum is a buffer offset for next reading
            }

            fileStream.Close(); //Again - this is not needed, just me being paranoid and explicitly releasing resources ASAP

            char[] chars = new char[buffer.Length / sizeof(char) + 1];
            System.Buffer.BlockCopy(buffer, 0, chars, 0, buffer.Length);
            fileContents = new string(chars);
        }
    }
    catch (IOException ex)
    {
        //THE FUNKY MAGIC - TO SEE IF THIS FILE REALLY IS LOCKED!!!
        if (IsFileLocked(ex))
        {
            // do something? 
        }
    }
    catch (Exception ex)
    {
    }
    finally
    {
    }

    return fileContents;
}

你自己试试吧:

byte[] output1 = Helper.ReadFileBytes(@"c:\temp\test.txt");
string output2 = Helper.ReadFileTextWithEncoding(@"c:\temp\test.txt");
string output3 = Helper.ReadFileTextNoEncoding(@"c:\temp\test.txt");

上面接受的答案遇到了一个问题，如果文件已被打开以使用FileShare写入。读取模式或如果文件具有只读属性，代码将无法工作。这个修改后的解决方案最可靠，需要记住两件事(对于公认的解决方案也是如此):

它将不适用于已以写共享模式打开的文件 这没有考虑到线程问题，所以你需要锁定它或单独处理线程问题。

记住上面的内容，这将检查文件是被写入锁定还是被阻止读取锁定:

public static bool FileLocked(string FileName)
{
    FileStream fs = null;

    try
    {
        // NOTE: This doesn't handle situations where file is opened for writing by another process but put into write shared mode, it will not throw an exception and won't show it as write locked
        fs = File.Open(FileName, FileMode.Open, FileAccess.ReadWrite, FileShare.None); // If we can't open file for reading and writing then it's locked by another process for writing
    }
    catch (UnauthorizedAccessException) // https://msdn.microsoft.com/en-us/library/y973b725(v=vs.110).aspx
    {
        // This is because the file is Read-Only and we tried to open in ReadWrite mode, now try to open in Read only mode
        try
        {
            fs = File.Open(FileName, FileMode.Open, FileAccess.Read, FileShare.None);
        }
        catch (Exception)
        {
            return true; // This file has been locked, we can't even open it to read
        }
    }
    catch (Exception)
    {
        return true; // This file has been locked
    }
    finally
    {
        if (fs != null)
            fs.Close();
    }
    return false;
}

retry_possibility:
//somecode here

try
{
    using(FileStream stream = file.Open(FileMode.Open, FileAccess.Read, FileShare.None))
    {
        stream.Close();
    }
    //write or open your file here
}
catch (IOException)
{
    DialogResult dialogResult = MessageBox.Show("This file is opened by you or another user. Please close it and press retry.\n"+ expFilePath, "File Locked", MessageBoxButtons.RetryCancel);
    if (dialogResult == DialogResult.Retry)
    {
        goto retry_possibility;
    }
    else if (dialogResult == DialogResult.Cancel)
    {
        //do nothing
    }
}

static bool FileInUse(string path)
    {
        try
        {
            using (FileStream fs = new FileStream(path, FileMode.OpenOrCreate))
            {
                fs.CanWrite
            }
            return false;
        }
        catch (IOException ex)
        {
            return true;
        }
    }

string filePath = "C:\\Documents And Settings\\yourfilename";
bool isFileInUse;

isFileInUse = FileInUse(filePath);

// Then you can do some checking
if (isFileInUse)
   Console.WriteLine("File is in use");
else
   Console.WriteLine("File is not in use");

希望这能有所帮助!

我最近遇到了这个问题，找到了这个:https://learn.microsoft.com/en-us/dotnet/standard/io/handling-io-errors。

在这里，微软描述了以下检查IOException是否由于锁定文件导致的方法:

catch (IOException e) when ((e.HResult & 0x0000FFFF) == 32 ) {
    Console.WriteLine("There is a sharing violation.");
}