static bool FileInUse(string path)
    {
        try
        {
            using (FileStream fs = new FileStream(path, FileMode.OpenOrCreate))
            {
                fs.CanWrite
            }
            return false;
        }
        catch (IOException ex)
        {
            return true;
        }
    }

string filePath = "C:\\Documents And Settings\\yourfilename";
bool isFileInUse;

isFileInUse = FileInUse(filePath);

// Then you can do some checking
if (isFileInUse)
   Console.WriteLine("File is in use");
else
   Console.WriteLine("File is not in use");

希望这能有所帮助!

除了工作3-liner，只是为了参考:如果你想要完整的信息-在微软开发中心有一个小项目:

https://code.msdn.microsoft.com/windowsapps/How-to-know-the-process-704839f4

现发现于: https://github.com/TacticalHorse/LockFinder/blob/master/LockFinder.cs

引言:

The C# sample code developed in .NET Framework 4.0 would help in finding out which is the process that is having a lock on a file. RmStartSession function which is included in rstrtmgr.dll has been used to create a restart manager session and according to the return result a new instance of Win32Exception object is created. After registering the resources to a Restart Manager session via RmRegisterRescources function, RmGetList function is invoked to check what are the applications are using a particular file by enumerating the RM_PROCESS_INFO array.

它通过连接到“重启管理器会话”来工作。

重启管理器使用向会话注册的资源列表 确定哪些应用程序和服务必须关闭并重新启动。 资源可以通过文件名、服务短名称或 描述正在运行的应用程序的RM_UNIQUE_PROCESS结构。

为了满足你的特殊需求，它可能有点过度设计了…… 但如果这是你想要的，那就去拿vs-project吧。

尝试将文件移动/复制到临时目录。如果可以的话，它没有锁，您可以安全地在临时目录中工作而不需要锁。否则就在x秒内再移动一次。

使用此命令检查文件是否被锁定:

using System.IO;
using System.Runtime.InteropServices;
internal static class Helper
{
const int ERROR_SHARING_VIOLATION = 32;
const int ERROR_LOCK_VIOLATION = 33;

private static bool IsFileLocked(Exception exception)
{
    int errorCode = Marshal.GetHRForException(exception) & ((1 << 16) - 1);
    return errorCode == ERROR_SHARING_VIOLATION || errorCode == ERROR_LOCK_VIOLATION;
}

internal static bool CanReadFile(string filePath)
{
    //Try-Catch so we dont crash the program and can check the exception
    try {
        //The "using" is important because FileStream implements IDisposable and
        //"using" will avoid a heap exhaustion situation when too many handles  
        //are left undisposed.
        using (FileStream fileStream = File.Open(filePath, FileMode.Open, FileAccess.ReadWrite, FileShare.None)) {
            if (fileStream != null) fileStream.Close();  //This line is me being overly cautious, fileStream will never be null unless an exception occurs... and I know the "using" does it but its helpful to be explicit - especially when we encounter errors - at least for me anyway!
        }
    }
    catch (IOException ex) {
        //THE FUNKY MAGIC - TO SEE IF THIS FILE REALLY IS LOCKED!!!
        if (IsFileLocked(ex)) {
            // do something, eg File.Copy or present the user with a MsgBox - I do not recommend Killing the process that is locking the file
            return false;
        }
    }
    finally
    { }
    return true;
}
}

出于性能考虑，我建议您在同一操作中读取文件内容。下面是一些例子:

public static byte[] ReadFileBytes(string filePath)
{
    byte[] buffer = null;
    try
    {
        using (FileStream fileStream = File.Open(filePath, FileMode.Open, FileAccess.ReadWrite, FileShare.None))
        {
            int length = (int)fileStream.Length;  // get file length
            buffer = new byte[length];            // create buffer
            int count;                            // actual number of bytes read
            int sum = 0;                          // total number of bytes read

            // read until Read method returns 0 (end of the stream has been reached)
            while ((count = fileStream.Read(buffer, sum, length - sum)) > 0)
                sum += count;  // sum is a buffer offset for next reading

            fileStream.Close(); //This is not needed, just me being paranoid and explicitly releasing resources ASAP
        }
    }
    catch (IOException ex)
    {
        //THE FUNKY MAGIC - TO SEE IF THIS FILE REALLY IS LOCKED!!!
        if (IsFileLocked(ex))
        {
            // do something? 
        }
    }
    catch (Exception ex)
    {
    }
    finally
    {
    }
    return buffer;
}

public static string ReadFileTextWithEncoding(string filePath)
{
    string fileContents = string.Empty;
    byte[] buffer;
    try
    {
        using (FileStream fileStream = File.Open(filePath, FileMode.Open, FileAccess.ReadWrite, FileShare.None))
        {
            int length = (int)fileStream.Length;  // get file length
            buffer = new byte[length];            // create buffer
            int count;                            // actual number of bytes read
            int sum = 0;                          // total number of bytes read

            // read until Read method returns 0 (end of the stream has been reached)
            while ((count = fileStream.Read(buffer, sum, length - sum)) > 0)
            {
                sum += count;  // sum is a buffer offset for next reading
            }

            fileStream.Close(); //Again - this is not needed, just me being paranoid and explicitly releasing resources ASAP

            //Depending on the encoding you wish to use - I'll leave that up to you
            fileContents = System.Text.Encoding.Default.GetString(buffer);
        }
    }
    catch (IOException ex)
    {
        //THE FUNKY MAGIC - TO SEE IF THIS FILE REALLY IS LOCKED!!!
        if (IsFileLocked(ex))
        {
            // do something? 
        }
    }
    catch (Exception ex)
    {
    }
    finally
    { }     
    return fileContents;
}

public static string ReadFileTextNoEncoding(string filePath)
{
    string fileContents = string.Empty;
    byte[] buffer;
    try
    {
        using (FileStream fileStream = File.Open(filePath, FileMode.Open, FileAccess.ReadWrite, FileShare.None))
        {
            int length = (int)fileStream.Length;  // get file length
            buffer = new byte[length];            // create buffer
            int count;                            // actual number of bytes read
            int sum = 0;                          // total number of bytes read

            // read until Read method returns 0 (end of the stream has been reached)
            while ((count = fileStream.Read(buffer, sum, length - sum)) > 0) 
            {
                sum += count;  // sum is a buffer offset for next reading
            }

            fileStream.Close(); //Again - this is not needed, just me being paranoid and explicitly releasing resources ASAP

            char[] chars = new char[buffer.Length / sizeof(char) + 1];
            System.Buffer.BlockCopy(buffer, 0, chars, 0, buffer.Length);
            fileContents = new string(chars);
        }
    }
    catch (IOException ex)
    {
        //THE FUNKY MAGIC - TO SEE IF THIS FILE REALLY IS LOCKED!!!
        if (IsFileLocked(ex))
        {
            // do something? 
        }
    }
    catch (Exception ex)
    {
    }
    finally
    {
    }

    return fileContents;
}

你自己试试吧:

byte[] output1 = Helper.ReadFileBytes(@"c:\temp\test.txt");
string output2 = Helper.ReadFileTextWithEncoding(@"c:\temp\test.txt");
string output3 = Helper.ReadFileTextNoEncoding(@"c:\temp\test.txt");

根据我的经验，你通常想这样做，然后“保护”你的文件做一些奇特的事情，然后使用“保护”文件。如果你只想这样使用一个文件，你可以使用Jeremy Thompson在回答中解释的技巧。但是，如果您试图对大量文件执行此操作(例如，当您正在编写安装程序时)，则会受到相当大的伤害。

解决这个问题的一个非常优雅的方法是，如果您的文件系统中有一个文件正在使用，那么它将不允许您更改文件夹名称。将文件夹保存在同一个文件系统中，它会非常有效。

请注意，您应该了解可以利用这一点的明显方式。毕竟，文件不会被锁定。此外，请注意还有其他原因可能导致Move操作失败。显然，适当的错误处理(MSDN)可以帮助解决这个问题。

var originalFolder = @"c:\myHugeCollectionOfFiles"; // your folder name here
var someFolder = Path.Combine(originalFolder, "..", Guid.NewGuid().ToString("N"));

try
{
    Directory.Move(originalFolder, someFolder);

    // Use files
}
catch // TODO: proper exception handling
{
    // Inform user, take action
}
finally
{
    Directory.Move(someFolder, originalFolder);
}

对于单个文件，我坚持使用Jeremy Thompson发布的锁定建议。

更新了该解决方案的说明:使用FileAccess检查。对于只读文件，ReadWrite将失败，因此解决方案已修改为使用FileAccess.Read进行检查。

原: 在过去的几年里，我一直在使用这段代码，没有遇到过任何问题。

理解你在使用异常时的犹豫，但你不能总是避免它们:

protected virtual bool IsFileLocked(FileInfo file)
{
    try
    {
        using(FileStream stream = file.Open(FileMode.Open, FileAccess.Read, FileShare.None))
        {
            stream.Close();
        }
    }
    catch (IOException)
    {
        //the file is unavailable because it is:
        //still being written to
        //or being processed by another thread
        //or does not exist (has already been processed)
        return true;
    }

    //file is not locked
    return false;
}