I think the right answer is a combination of Steve Morgan's and Serguei's answers. That's how Internet Explorer does it. The pinvoke call to FindMimeFromData works for only 26 hard-coded mime types. Also, it will give ambigous mime types (such as text/plain or application/octet-stream) even though there may exist a more specific, more appropriate mime type. If it fails to give a good mime type, you can go to the registry for a more specific mime type. The server registry could have more up-to-date mime types.

参考网址:http://msdn.microsoft.com/en-us/library/ms775147(VS.85).aspx

如果你正在使用。net Framework 4.5或更高版本，现在有一个MimeMapping.GetMimeMapping(filename)方法，它将返回一个字符串，其中包含传递文件名的正确Mime映射。注意，这里使用的是文件扩展名，而不是文件本身中的数据。

文档请见http://msdn.microsoft.com/en-us/library/system.web.mimemapping.getmimemapping

如果有人对此感兴趣，他们可以将优秀的perl模块File::Type移植到。net。在代码中是一组文件头魔术数字查找每个文件类型或正则表达式匹配。

这里有一个。net文件类型检测库http://filetypedetective.codeplex.com/，但它目前只检测少量的文件。

iis7或以上

使用此代码，但您需要是服务器上的管理员

public bool CheckMimeMapExtension(string fileExtension)
        {
            try
            {

                using (
                ServerManager serverManager = new ServerManager())
                {   
                    // connects to default app.config
                    var config = serverManager.GetApplicationHostConfiguration();
                    var staticContent = config.GetSection("system.webServer/staticContent");
                    var mimeMap = staticContent.GetCollection();

                    foreach (var mimeType in mimeMap)
                    {

                        if (((String)mimeType["fileExtension"]).Equals(fileExtension, StringComparison.OrdinalIgnoreCase))
                            return true;

                    }

                }
                return false;
            }
            catch (Exception ex)
            { 
                Console.WriteLine("An exception has occurred: \n{0}", ex.Message);
                Console.Read();
            }

            return false;

        }

我使用混合解决方案:

    using System.Runtime.InteropServices;

    [DllImport (@"urlmon.dll", CharSet = CharSet.Auto)]
    private extern static System.UInt32 FindMimeFromData(
        System.UInt32 pBC, 
        [MarshalAs(UnmanagedType.LPStr)] System.String pwzUrl,
        [MarshalAs(UnmanagedType.LPArray)] byte[] pBuffer,
        System.UInt32 cbSize,
        [MarshalAs(UnmanagedType.LPStr)] System.String pwzMimeProposed,
        System.UInt32 dwMimeFlags,
        out System.UInt32 ppwzMimeOut,
        System.UInt32 dwReserverd
    );

    private string GetMimeFromRegistry (string Filename)
    {
        string mime = "application/octetstream";
        string ext = System.IO.Path.GetExtension(Filename).ToLower();
        Microsoft.Win32.RegistryKey rk = Microsoft.Win32.Registry.ClassesRoot.OpenSubKey(ext);
        if (rk != null && rk.GetValue("Content Type") != null)
            mime = rk.GetValue("Content Type").ToString();
        return mime;
    }

    public string GetMimeTypeFromFileAndRegistry (string filename)
    {
        if (!File.Exists(filename))
        {
           return GetMimeFromRegistry (filename);
        }

        byte[] buffer = new byte[256];

        using (FileStream fs = new FileStream(filename, FileMode.Open))
        {
            if (fs.Length >= 256)
                fs.Read(buffer, 0, 256);
            else
                fs.Read(buffer, 0, (int)fs.Length);
        }

        try
        {            
            System.UInt32 mimetype;

            FindMimeFromData(0, null, buffer, 256, null, 0, out mimetype, 0);

            System.IntPtr mimeTypePtr = new IntPtr(mimetype);

            string mime = Marshal.PtrToStringUni(mimeTypePtr);

            Marshal.FreeCoTaskMem(mimeTypePtr);

            if (string.IsNullOrWhiteSpace (mime) || 
                mime =="text/plain" || mime == "application/octet-stream")                    
            {
                return GetMimeFromRegistry (filename);
            }

            return mime;
        }
        catch (Exception e)
        {
            return GetMimeFromRegistry (filename);
        }
    }

编辑:只需使用Mime侦探

我使用字节数组序列来确定给定文件的正确MIME类型。与只查看文件名的文件扩展名相比，这样做的好处是，如果用户要重命名一个文件以绕过某些文件类型的上传限制，则文件扩展名将无法捕捉到这一点。另一方面，通过字节数组获取文件签名将阻止这种有害行为的发生。

下面是c#中的一个例子:

public class MimeType
{
    private static readonly byte[] BMP = { 66, 77 };
    private static readonly byte[] DOC = { 208, 207, 17, 224, 161, 177, 26, 225 };
    private static readonly byte[] EXE_DLL = { 77, 90 };
    private static readonly byte[] GIF = { 71, 73, 70, 56 };
    private static readonly byte[] ICO = { 0, 0, 1, 0 };
    private static readonly byte[] JPG = { 255, 216, 255 };
    private static readonly byte[] MP3 = { 255, 251, 48 };
    private static readonly byte[] OGG = { 79, 103, 103, 83, 0, 2, 0, 0, 0, 0, 0, 0, 0, 0 };
    private static readonly byte[] PDF = { 37, 80, 68, 70, 45, 49, 46 };
    private static readonly byte[] PNG = { 137, 80, 78, 71, 13, 10, 26, 10, 0, 0, 0, 13, 73, 72, 68, 82 };
    private static readonly byte[] RAR = { 82, 97, 114, 33, 26, 7, 0 };
    private static readonly byte[] SWF = { 70, 87, 83 };
    private static readonly byte[] TIFF = { 73, 73, 42, 0 };
    private static readonly byte[] TORRENT = { 100, 56, 58, 97, 110, 110, 111, 117, 110, 99, 101 };
    private static readonly byte[] TTF = { 0, 1, 0, 0, 0 };
    private static readonly byte[] WAV_AVI = { 82, 73, 70, 70 };
    private static readonly byte[] WMV_WMA = { 48, 38, 178, 117, 142, 102, 207, 17, 166, 217, 0, 170, 0, 98, 206, 108 };
    private static readonly byte[] ZIP_DOCX = { 80, 75, 3, 4 };

    public static string GetMimeType(byte[] file, string fileName)
    {

        string mime = "application/octet-stream"; //DEFAULT UNKNOWN MIME TYPE

        //Ensure that the filename isn't empty or null
        if (string.IsNullOrWhiteSpace(fileName))
        {
            return mime;
        }

        //Get the file extension
        string extension = Path.GetExtension(fileName) == null
                               ? string.Empty
                               : Path.GetExtension(fileName).ToUpper();

        //Get the MIME Type
        if (file.Take(2).SequenceEqual(BMP))
        {
            mime = "image/bmp";
        }
        else if (file.Take(8).SequenceEqual(DOC))
        {
            mime = "application/msword";
        }
        else if (file.Take(2).SequenceEqual(EXE_DLL))
        {
            mime = "application/x-msdownload"; //both use same mime type
        }
        else if (file.Take(4).SequenceEqual(GIF))
        {
            mime = "image/gif";
        }
        else if (file.Take(4).SequenceEqual(ICO))
        {
            mime = "image/x-icon";
        }
        else if (file.Take(3).SequenceEqual(JPG))
        {
            mime = "image/jpeg";
        }
        else if (file.Take(3).SequenceEqual(MP3))
        {
            mime = "audio/mpeg";
        }
        else if (file.Take(14).SequenceEqual(OGG))
        {
            if (extension == ".OGX")
            {
                mime = "application/ogg";
            }
            else if (extension == ".OGA")
            {
                mime = "audio/ogg";
            }
            else
            {
                mime = "video/ogg";
            }
        }
        else if (file.Take(7).SequenceEqual(PDF))
        {
            mime = "application/pdf";
        }
        else if (file.Take(16).SequenceEqual(PNG))
        {
            mime = "image/png";
        }
        else if (file.Take(7).SequenceEqual(RAR))
        {
            mime = "application/x-rar-compressed";
        }
        else if (file.Take(3).SequenceEqual(SWF))
        {
            mime = "application/x-shockwave-flash";
        }
        else if (file.Take(4).SequenceEqual(TIFF))
        {
            mime = "image/tiff";
        }
        else if (file.Take(11).SequenceEqual(TORRENT))
        {
            mime = "application/x-bittorrent";
        }
        else if (file.Take(5).SequenceEqual(TTF))
        {
            mime = "application/x-font-ttf";
        }
        else if (file.Take(4).SequenceEqual(WAV_AVI))
        {
            mime = extension == ".AVI" ? "video/x-msvideo" : "audio/x-wav";
        }
        else if (file.Take(16).SequenceEqual(WMV_WMA))
        {
            mime = extension == ".WMA" ? "audio/x-ms-wma" : "video/x-ms-wmv";
        }
        else if (file.Take(4).SequenceEqual(ZIP_DOCX))
        {
            mime = extension == ".DOCX" ? "application/vnd.openxmlformats-officedocument.wordprocessingml.document" : "application/x-zip-compressed";
        }

        return mime;
    }


}

注意，我对DOCX文件类型的处理是不同的，因为DOCX实际上只是一个ZIP文件。在这个场景中，只要验证了文件扩展名的顺序，我就会检查文件扩展名。对于某些人来说，这个示例还远远不够完整，但是您可以轻松地添加自己的示例。

如果您想添加更多MIME类型，您可以从这里获得许多不同文件类型的字节数组序列。另外，这里还有一个关于文件签名的很好的资源。

如果其他方法都失败了，我经常做的是逐步查看我正在寻找的特定类型的几个文件，并在文件的字节序列中寻找模式。最后，这仍然是基本的验证，不能用于确定文件类型的100%证明。