这将使你能够播放声音多次，与投票最多的答案形成对比:

var playSound = (function beep() {
    var snd = new Audio("data:audio/wav;base64,//uQRAAAAWMSLwUIYAAsYkXgoQwAEaYLWfkWgAI0wWs/ItAAAGDgYtAgAyN+QWaAAihwMWm4G8QQRDiMcCBcH3Cc+CDv/7xA4Tvh9Rz/y8QADBwMWgQAZG/ILNAARQ4GLTcDeIIIhxGOBAuD7hOfBB3/94gcJ3w+o5/5eIAIAAAVwWgQAVQ2ORaIQwEMAJiDg95G4nQL7mQVWI6GwRcfsZAcsKkJvxgxEjzFUgfHoSQ9Qq7KNwqHwuB13MA4a1q/DmBrHgPcmjiGoh//EwC5nGPEmS4RcfkVKOhJf+WOgoxJclFz3kgn//dBA+ya1GhurNn8zb//9NNutNuhz31f////9vt///z+IdAEAAAK4LQIAKobHItEIYCGAExBwe8jcToF9zIKrEdDYIuP2MgOWFSE34wYiR5iqQPj0JIeoVdlG4VD4XA67mAcNa1fhzA1jwHuTRxDUQ//iYBczjHiTJcIuPyKlHQkv/LHQUYkuSi57yQT//uggfZNajQ3Vmz+Zt//+mm3Wm3Q576v////+32///5/EOgAAADVghQAAAAA//uQZAUAB1WI0PZugAAAAAoQwAAAEk3nRd2qAAAAACiDgAAAAAAABCqEEQRLCgwpBGMlJkIz8jKhGvj4k6jzRnqasNKIeoh5gI7BJaC1A1AoNBjJgbyApVS4IDlZgDU5WUAxEKDNmmALHzZp0Fkz1FMTmGFl1FMEyodIavcCAUHDWrKAIA4aa2oCgILEBupZgHvAhEBcZ6joQBxS76AgccrFlczBvKLC0QI2cBoCFvfTDAo7eoOQInqDPBtvrDEZBNYN5xwNwxQRfw8ZQ5wQVLvO8OYU+mHvFLlDh05Mdg7BT6YrRPpCBznMB2r//xKJjyyOh+cImr2/4doscwD6neZjuZR4AgAABYAAAABy1xcdQtxYBYYZdifkUDgzzXaXn98Z0oi9ILU5mBjFANmRwlVJ3/6jYDAmxaiDG3/6xjQQCCKkRb/6kg/wW+kSJ5//rLobkLSiKmqP/0ikJuDaSaSf/6JiLYLEYnW/+kXg1WRVJL/9EmQ1YZIsv/6Qzwy5qk7/+tEU0nkls3/zIUMPKNX/6yZLf+kFgAfgGyLFAUwY//uQZAUABcd5UiNPVXAAAApAAAAAE0VZQKw9ISAAACgAAAAAVQIygIElVrFkBS+Jhi+EAuu+lKAkYUEIsmEAEoMeDmCETMvfSHTGkF5RWH7kz/ESHWPAq/kcCRhqBtMdokPdM7vil7RG98A2sc7zO6ZvTdM7pmOUAZTnJW+NXxqmd41dqJ6mLTXxrPpnV8avaIf5SvL7pndPvPpndJR9Kuu8fePvuiuhorgWjp7Mf/PRjxcFCPDkW31srioCExivv9lcwKEaHsf/7ow2Fl1T/9RkXgEhYElAoCLFtMArxwivDJJ+bR1HTKJdlEoTELCIqgEwVGSQ+hIm0NbK8WXcTEI0UPoa2NbG4y2K00JEWbZavJXkYaqo9CRHS55FcZTjKEk3NKoCYUnSQ0rWxrZbFKbKIhOKPZe1cJKzZSaQrIyULHDZmV5K4xySsDRKWOruanGtjLJXFEmwaIbDLX0hIPBUQPVFVkQkDoUNfSoDgQGKPekoxeGzA4DUvnn4bxzcZrtJyipKfPNy5w+9lnXwgqsiyHNeSVpemw4bWb9psYeq//uQZBoABQt4yMVxYAIAAAkQoAAAHvYpL5m6AAgAACXDAAAAD59jblTirQe9upFsmZbpMudy7Lz1X1DYsxOOSWpfPqNX2WqktK0DMvuGwlbNj44TleLPQ+Gsfb+GOWOKJoIrWb3cIMeeON6lz2umTqMXV8Mj30yWPpjoSa9ujK8SyeJP5y5mOW1D6hvLepeveEAEDo0mgCRClOEgANv3B9a6fikgUSu/DmAMATrGx7nng5p5iimPNZsfQLYB2sDLIkzRKZOHGAaUyDcpFBSLG9MCQALgAIgQs2YunOszLSAyQYPVC2YdGGeHD2dTdJk1pAHGAWDjnkcLKFymS3RQZTInzySoBwMG0QueC3gMsCEYxUqlrcxK6k1LQQcsmyYeQPdC2YfuGPASCBkcVMQQqpVJshui1tkXQJQV0OXGAZMXSOEEBRirXbVRQW7ugq7IM7rPWSZyDlM3IuNEkxzCOJ0ny2ThNkyRai1b6ev//3dzNGzNb//4uAvHT5sURcZCFcuKLhOFs8mLAAEAt4UWAAIABAAAAAB4qbHo0tIjVkUU//uQZAwABfSFz3ZqQAAAAAngwAAAE1HjMp2qAAAAACZDgAAAD5UkTE1UgZEUExqYynN1qZvqIOREEFmBcJQkwdxiFtw0qEOkGYfRDifBui9MQg4QAHAqWtAWHoCxu1Yf4VfWLPIM2mHDFsbQEVGwyqQoQcwnfHeIkNt9YnkiaS1oizycqJrx4KOQjahZxWbcZgztj2c49nKmkId44S71j0c8eV9yDK6uPRzx5X18eDvjvQ6yKo9ZSS6l//8elePK/Lf//IInrOF/FvDoADYAGBMGb7FtErm5MXMlmPAJQVgWta7Zx2go+8xJ0UiCb8LHHdftWyLJE0QIAIsI+UbXu67dZMjmgDGCGl1H+vpF4NSDckSIkk7Vd+sxEhBQMRU8j/12UIRhzSaUdQ+rQU5kGeFxm+hb1oh6pWWmv3uvmReDl0UnvtapVaIzo1jZbf/pD6ElLqSX+rUmOQNpJFa/r+sa4e/pBlAABoAAAAA3CUgShLdGIxsY7AUABPRrgCABdDuQ5GC7DqPQCgbbJUAoRSUj+NIEig0YfyWUho1VBBBA//uQZB4ABZx5zfMakeAAAAmwAAAAF5F3P0w9GtAAACfAAAAAwLhMDmAYWMgVEG1U0FIGCBgXBXAtfMH10000EEEEEECUBYln03TTTdNBDZopopYvrTTdNa325mImNg3TTPV9q3pmY0xoO6bv3r00y+IDGid/9aaaZTGMuj9mpu9Mpio1dXrr5HERTZSmqU36A3CumzN/9Robv/Xx4v9ijkSRSNLQhAWumap82WRSBUqXStV/YcS+XVLnSS+WLDroqArFkMEsAS+eWmrUzrO0oEmE40RlMZ5+ODIkAyKAGUwZ3mVKmcamcJnMW26MRPgUw6j+LkhyHGVGYjSUUKNpuJUQoOIAyDvEyG8S5yfK6dhZc0Tx1KI/gviKL6qvvFs1+bWtaz58uUNnryq6kt5RzOCkPWlVqVX2a/EEBUdU1KrXLf40GoiiFXK///qpoiDXrOgqDR38JB0bw7SoL+ZB9o1RCkQjQ2CBYZKd/+VJxZRRZlqSkKiws0WFxUyCwsKiMy7hUVFhIaCrNQsKkTIsLivwKKigsj8XYlwt/WKi2N4d//uQRCSAAjURNIHpMZBGYiaQPSYyAAABLAAAAAAAACWAAAAApUF/Mg+0aohSIRobBAsMlO//Kk4soosy1JSFRYWaLC4qZBYWFRGZdwqKiwkNBVmoWFSJkWFxX4FFRQWR+LsS4W/rFRb/////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////VEFHAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAU291bmRib3kuZGUAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAMjAwNGh0dHA6Ly93d3cuc291bmRib3kuZGUAAAAAAAAAACU=");  
    return function() {     
        snd.play(); 
    }
})();

playSound(); // Play first time
playSound(); // Play second time

<html>
<head>
    <script src='https://surikov.github.io/webaudiofont/npm/dist/WebAudioFontPlayer.js'></script>
    <script src='https://surikov.github.io/webaudiofontdata/sound/0000_JCLive_sf2_file.js'></script>
    <script>
        var selectedPreset=_tone_0000_JCLive_sf2_file;
        var AudioContextFunc = window.AudioContext || window.webkitAudioContext;
        var audioContext = new AudioContextFunc();
        var player=new WebAudioFontPlayer();
        player.loader.decodeAfterLoading(audioContext, '_tone_0000_JCLive_sf2_file');
    </script>
</head>
<body>
    <p><a href="#" onmousedown="player.queueWaveTable(audioContext, audioContext.destination, selectedPreset, 0, 55, 3.5);">Play a note</a></p>
    <hr/>
    <p><a href="https://github.com/surikov/webaudiofont">source</a></p>
</body>

听这里 https://jsbin.com/lamidog/1/edit?html,output

该代码支持多个哔哔声的排序，以及频率的逐渐变化('ramp'标志)。下面给出了两个例子。

<script>
var audioContext = null;
var oscillatorNode = null;
var stopTime = 0;

function beep (frequency, durationSec, ramp=false)
{
    if (oscillatorNode == null) {
        audioContext = new (window.AudioContext || window.webkitAudioContext) ();
        stopTime = audioContext.currentTime;

        oscillatorNode = audioContext.createOscillator();
        oscillatorNode.type = "sine";
        oscillatorNode.connect (audioContext.destination);
        if (ramp) {
            oscillatorNode.frequency.setValueAtTime (frequency, stopTime);
        }
        oscillatorNode.start ();
        oscillatorNode.onended = function() {
            oscillatorNode = null;
            audioContext = null;
        }
    }

    if (ramp) {
        oscillatorNode.frequency.linearRampToValueAtTime (frequency, stopTime); // value in hertz
    } else {
        oscillatorNode.frequency.setValueAtTime (frequency, stopTime);  // value in hertz
    }

    stopTime += durationSec;
    oscillatorNode.stop (stopTime);
}
function test1()
{
        beep (250, 0.5);
        beep (1000, 0.2);
        beep (550, 0.5);
}
    
function test2()
{
        beep (50, 2, true);
        beep (5000, 2, true);
        beep (50, 0, true);
}
</script>
    
<button onclick='test1()'>Beep!</button>
<button onclick='test2()'>Beep(ramped)!</button>

当我们读到这个答案时，HTML5将为你解决这个问题，如果你愿意这样做的话。所有现代浏览器都支持HTML5音频。

下面是示例的副本:

var snd = new Audio("file.wav"); // buffers automatically when created
snd.play();

function beep(freq = 660, duration = 90, vol = 50) { var context = new(window.AudioContext || window.webkitAudioContext); const oscillator = context.createOscillator(); const gain = context.createGain(); gain.gain.setValueAtTime(0, context.currentTime); gain.gain.linearRampToValueAtTime(1, context.currentTime + 0.002); oscillator.connect(gain); oscillator.frequency.value = freq; oscillator.type = "square"; gain.connect(context.destination); oscillator.start(context.currentTime); oscillator.stop(context.currentTime + duration * .001); oscillator.onended = () => context.close(); } <br> <center><button onclick="beep()">Beep!</button></center>

现在用JavaScript AudioContext API就很简单了。主要的桌面和移动网络浏览器完全支持它…

let context = null;

const beep = (freq = 520, duration = 200, vol = 100) => {
    const oscillator = context.createOscillator();
    const gain = context.createGain();
    oscillator.connect(gain);
    oscillator.frequency.value = freq;
    oscillator.type = "square";
    gain.connect(context.destination);
    gain.gain.value = vol * 0.01;
    oscillator.start(context.currentTime);
    oscillator.stop(context.currentTime + duration * 0.001);
}

document.querySelector('button').addEventListener('click', function () {
    context = new AudioContext();
    beep();
});

我写了一个小应用程序，可以播放马里奥游戏中的音乐，不需要任何音频文件，只有运行时。在我看来，这很有趣，你可以看到源代码并听它。