我也得到了同样的错误…检查您的虚拟目录的IIS配置，并确保属性- ASP。Net - asp。NET版本与项目属性-应用程序-目标框架相同。(这为我解决了这个错误。)

我也面临着同样的问题。尽管遵循每一个答案，它没有工作。 然后我将“Inherits=namespace.class”更改为“Inherits=完全限定的组装名”，即“Inherits=namespace.class,assemblyname, Version=， Culture=， PublicKeyToken=” 希望能有所帮助。

我不得不去BUILD ->配置管理器，然后——咳咳——勾选我的项目旁边的复选框，以确保它实际上得到了构建。

TL;DR:对我来说，在尝试了3天我能在这里找到的所有其他答案之后，最终的解决方案是在我的IIS应用程序池高级设置中将启用32位应用程序设置为True，然后重新创建临时ASP。并为我的IIS_IUSRS组增加“修改”和“写入”权限。

我尝试的步骤:

Completely clean and rebuild solution. Shut down and restart Visual Studio. Delete Temporary ASP.NET Files directories (C:\Windows\Microsoft.NET\Framework*). This is where my problems really started: I deleted too much. Reboot development machine. Remove .vs folder and rebuild solution. Recreate C:\Windows\Microsoft.NET\Framework\v4.0.30319\Temporary ASP.NET Files. My projects are 64-bit applications, even though build platform is AnyCPU. Verify output directory was set to bin/ on web projects. Wipe out and check out a clean copy of my version control branch. Wipe out and clone a fresh copy of my version control repository. Run Windows update to latest versions of .NET Frameworks. Repair, uninstall, and reinstall .NET Frameworks 3.5 and 4.8. Uninstall and reinstall Visual Studio 2019 Professional. Uninstall and reinstall the Internet Information Services feature in Windows. Downgrade ASP.NET NuGet package and rebuild solution (issued reference errors due to changes in assemblies). Upgrade ASP.NET back to what my projects were using and rebuild solution. Recreate IIS virtual directory through web project's Web properties. Created "empty" Global.asax files. Restore my files after no change. Replace Codebehind="Global.asax.cs" with Codefile="Global.asax.cs". This started showing me load errors for references to assemblies that actually existed in the system. Enable 32-bit applications in IIS application pool. This started displaying an access error to a 32-bit temporary directory. Restore permissions to all Temporary ASP.NET Files directories (add all but Full Control). This finally got my web application working again.

我能够从网上找到的无数答案中拼凑出足够的信息。在搜索iis找不到引用的。net dll后找到了这个答案，我尝试了最后的几个步骤，让我再次工作，在我的第四天开始与这个令人难以置信的模糊问题作斗争。

如果有办法诊断出为什么全球。asax无法加载，而不是太通用的无法加载类型错误，没有详细信息。

这个故事的寓意:当清理临时ASP。NET Files目录，删除目录的内容，而不是目录本身。否则，可能会导致权限丢失。

“BUILD ->配置管理器和——嗯哼——勾选我的项目旁边的复选框，以确保它实际上得到了构建。” 然后在windows资源管理器中打开项目文件夹，按下选项并取消“只读”复选框。

Yes, I read all the answers. However, if you are me and have been pulling out all of what's left of your hair, then try checking the \bin folder. Like most proj files might have several configurations grouped under the XML element PropertyGroup, then I changed the OutputPath value from 'bin\Debug' to remove the '\Debug' part and Rebuild. This placed the files in the \bin folder allowing the Express IIS to find and load the build. I am left wondering what is the correct way to manage these different builds so that a local debug deploy is able to find and load the target environment.