使用windows7, python2.7, opencv 3.0，以下工作为我:

import cv2
import os

vvw           =   cv2.VideoWriter('mymovie.avi',cv2.VideoWriter_fourcc('X','V','I','D'),24,(640,480))
frameslist    =   os.listdir('.\\frames')
howmanyframes =   len(frameslist)
print('Frames count: '+str(howmanyframes)) #just for debugging

for i in range(0,howmanyframes):
    print(i)
    theframe = cv2.imread('.\\frames\\'+frameslist[i])
    vvw.write(theframe)

我使用images2gif.py，这很容易使用。它似乎是文件大小的两倍。

26个110kb的PNG文件，我期望26*110kb = 2860kb，但my_gif.GIF是5.7mb

另外，因为GIF是8位的，好看的png在GIF中变得有点模糊

下面是我使用的代码:

__author__ = 'Robert'
from images2gif import writeGif
from PIL import Image
import os

file_names = sorted((fn for fn in os.listdir('.') if fn.endswith('.png')))
#['animationframa.png', 'animationframb.png', 'animationframc.png', ...] "

images = [Image.open(fn) for fn in file_names]

print writeGif.__doc__
# writeGif(filename, images, duration=0.1, loops=0, dither=1)
#    Write an animated gif from the specified images.
#    images should be a list of numpy arrays of PIL images.
#    Numpy images of type float should have pixels between 0 and 1.
#    Numpy images of other types are expected to have values between 0 and 255.


#images.extend(reversed(images)) #infinit loop will go backwards and forwards.

filename = "my_gif.GIF"
writeGif(filename, images, duration=0.2)
#54 frames written
#
#Process finished with exit code 0

以下是26帧中的3帧:

缩小图像会减小尺寸:

size = (150,150)
for im in images:
    im.thumbnail(size, Image.ANTIALIAS)

就像沃伦去年说的，这是一个老问题。由于人们似乎仍然在浏览页面，我想将他们重定向到一个更现代的解决方案。就像blakev说的，github上有一个枕头的例子。

 import ImageSequence
 import Image
 import gifmaker
 sequence = []

 im = Image.open(....)

 # im is your original image
 frames = [frame.copy() for frame in ImageSequence.Iterator(im)]

 # write GIF animation
 fp = open("out.gif", "wb")
 gifmaker.makedelta(fp, frames)
 fp.close()

注意:这个例子已经过时了(gifmaker不是一个可导入的模块，只是一个脚本)。Pillow有一个GifImagePlugin(其源代码在GitHub上)，但ImageSequence上的文档似乎表明有限的支持(只读)

一个制作动图的简单函数:

import imageio
import pathlib
from datetime import datetime


def make_gif(image_directory: pathlib.Path, frames_per_second: float, **kwargs):
    """
    Makes a .gif which shows many images at a given frame rate.
    All images should be in order (don't know how this works) in the image directory

    Only tested with .png images but may work with others.

    :param image_directory:
    :type image_directory: pathlib.Path
    :param frames_per_second:
    :type frames_per_second: float
    :param kwargs: image_type='png' or other
    :return: nothing
    """
    assert isinstance(image_directory, pathlib.Path), "input must be a pathlib object"
    image_type = kwargs.get('type', 'png')

    timestampStr = datetime.now().strftime("%y%m%d_%H%M%S")
    gif_dir = image_directory.joinpath(timestampStr + "_GIF.gif")

    print('Started making GIF')
    print('Please wait... ')

    images = []
    for file_name in image_directory.glob('*.' + image_type):
        images.append(imageio.imread(image_directory.joinpath(file_name)))
    imageio.mimsave(gif_dir.as_posix(), images, fps=frames_per_second)

    print('Finished making GIF!')
    print('GIF can be found at: ' + gif_dir.as_posix())


def main():
    fps = 2
    png_dir = pathlib.Path('C:/temp/my_images')
    make_gif(png_dir, fps)

if __name__ == "__main__":
    main()

现在我在用ImageMagick。我将帧保存为PNG文件，然后从Python中调用ImageMagick的convert.exe来创建一个动画GIF。这种方法的好处是我可以为每一帧单独指定帧持续时间。不幸的是，这取决于ImageMagick安装在机器上。他们有一个Python包装器，但它看起来很糟糕，不受支持。还在考虑其他建议。

它不是一个python库，但mencoder可以做到这一点:从多个输入图像文件编码。你可以像这样从python中执行mencoder:

import os

os.system("mencoder ...")