使用windows7, python2.7, opencv 3.0，以下工作为我:

import cv2
import os

vvw           =   cv2.VideoWriter('mymovie.avi',cv2.VideoWriter_fourcc('X','V','I','D'),24,(640,480))
frameslist    =   os.listdir('.\\frames')
howmanyframes =   len(frameslist)
print('Frames count: '+str(howmanyframes)) #just for debugging

for i in range(0,howmanyframes):
    print(i)
    theframe = cv2.imread('.\\frames\\'+frameslist[i])
    vvw.write(theframe)

要创建视频，你可以使用opencv，

#load your frames
frames = ...
#create a video writer
writer = cvCreateVideoWriter(filename, -1, fps, frame_size, is_color=1)
#and write your frames in a loop if you want
cvWriteFrame(writer, frames[i])

就像沃伦去年说的，这是一个老问题。由于人们似乎仍然在浏览页面，我想将他们重定向到一个更现代的解决方案。就像blakev说的，github上有一个枕头的例子。

 import ImageSequence
 import Image
 import gifmaker
 sequence = []

 im = Image.open(....)

 # im is your original image
 frames = [frame.copy() for frame in ImageSequence.Iterator(im)]

 # write GIF animation
 fp = open("out.gif", "wb")
 gifmaker.makedelta(fp, frames)
 fp.close()

注意:这个例子已经过时了(gifmaker不是一个可导入的模块，只是一个脚本)。Pillow有一个GifImagePlugin(其源代码在GitHub上)，但ImageSequence上的文档似乎表明有限的支持(只读)

它不是一个python库，但mencoder可以做到这一点:从多个输入图像文件编码。你可以像这样从python中执行mencoder:

import os

os.system("mencoder ...")

一个制作动图的简单函数:

import imageio
import pathlib
from datetime import datetime


def make_gif(image_directory: pathlib.Path, frames_per_second: float, **kwargs):
    """
    Makes a .gif which shows many images at a given frame rate.
    All images should be in order (don't know how this works) in the image directory

    Only tested with .png images but may work with others.

    :param image_directory:
    :type image_directory: pathlib.Path
    :param frames_per_second:
    :type frames_per_second: float
    :param kwargs: image_type='png' or other
    :return: nothing
    """
    assert isinstance(image_directory, pathlib.Path), "input must be a pathlib object"
    image_type = kwargs.get('type', 'png')

    timestampStr = datetime.now().strftime("%y%m%d_%H%M%S")
    gif_dir = image_directory.joinpath(timestampStr + "_GIF.gif")

    print('Started making GIF')
    print('Please wait... ')

    images = []
    for file_name in image_directory.glob('*.' + image_type):
        images.append(imageio.imread(image_directory.joinpath(file_name)))
    imageio.mimsave(gif_dir.as_posix(), images, fps=frames_per_second)

    print('Finished making GIF!')
    print('GIF can be found at: ' + gif_dir.as_posix())


def main():
    fps = 2
    png_dir = pathlib.Path('C:/temp/my_images')
    make_gif(png_dir, fps)

if __name__ == "__main__":
    main()

Smart Manoj回答:从文件夹中的所有图像制作一个.mp4电影

安装:

pip install imageio-ffmpeg
pip install imageio

代码:

import os
import imageio

root = r'path_to_folder_with_images'

images = []    
for subdir, dirs, files in os.walk(root):
    for file in files:
        images.append(imageio.imread(os.path.join(root,file)))

savepath = r'path_to_save_folder'
imageio.mimsave(os.path.join(savepath,'movie.mp4'), images)

PS:确保你的“文件”列表按照你想要的方式排序，如果你已经相应地保存了你的图像，你会节省一些时间