使用windows7, python2.7, opencv 3.0，以下工作为我:

import cv2
import os

vvw           =   cv2.VideoWriter('mymovie.avi',cv2.VideoWriter_fourcc('X','V','I','D'),24,(640,480))
frameslist    =   os.listdir('.\\frames')
howmanyframes =   len(frameslist)
print('Frames count: '+str(howmanyframes)) #just for debugging

for i in range(0,howmanyframes):
    print(i)
    theframe = cv2.imread('.\\frames\\'+frameslist[i])
    vvw.write(theframe)

我偶然发现了PIL的ImageSequence模块，它提供了更好(更标准)的GIF动画。这次我也使用了Tk的after()方法，这比time.sleep()更好。

from Tkinter import * 
from PIL import Image, ImageTk, ImageSequence

def stop(event):
  global play
  play = False
  exit() 

root = Tk()
root.bind("<Key>", stop) # Press any key to stop
GIFfile = {path_to_your_GIF_file}
im = Image.open(GIFfile); img = ImageTk.PhotoImage(im)
delay = im.info['duration'] # Delay used in the GIF file 
lbl = Label(image=img); lbl.pack() # Create a label where to display images
play = True;
while play:
  for frame in ImageSequence.Iterator(im):
    if not play: break 
    root.after(delay);
    img = ImageTk.PhotoImage(frame)
    lbl.config(image=img); root.update() # Show the new frame/image

root.mainloop()

老问题，有很多很好的答案，但可能仍然对另一种选择感兴趣……

我最近在github (https://github.com/WarrenWeckesser/numpngw)上发布的numpngw模块可以从numpy数组写入动画PNG文件。(更新:numpngw现在在pypi: https://pypi.python.org/pypi/numpngw。)

例如，这个脚本:

import numpy as np
import numpngw


img0 = np.zeros((64, 64, 3), dtype=np.uint8)
img0[:32, :32, :] = 255
img1 = np.zeros((64, 64, 3), dtype=np.uint8)
img1[32:, :32, 0] = 255
img2 = np.zeros((64, 64, 3), dtype=np.uint8)
img2[32:, 32:, 1] = 255
img3 = np.zeros((64, 64, 3), dtype=np.uint8)
img3[:32, 32:, 2] = 255
seq = [img0, img1, img2, img3]
for img in seq:
    img[16:-16, 16:-16] = 127
    img[0, :] = 127
    img[-1, :] = 127
    img[:, 0] = 127
    img[:, -1] = 127

numpngw.write_apng('foo.png', seq, delay=250, use_palette=True)

创建:

你需要一个支持动画PNG的浏览器(直接或带有插件)才能看到动画。

它不是一个python库，但mencoder可以做到这一点:从多个输入图像文件编码。你可以像这样从python中执行mencoder:

import os

os.system("mencoder ...")

现在我在用ImageMagick。我将帧保存为PNG文件，然后从Python中调用ImageMagick的convert.exe来创建一个动画GIF。这种方法的好处是我可以为每一帧单独指定帧持续时间。不幸的是，这取决于ImageMagick安装在机器上。他们有一个Python包装器，但它看起来很糟糕，不受支持。还在考虑其他建议。

一个制作动图的简单函数:

import imageio
import pathlib
from datetime import datetime


def make_gif(image_directory: pathlib.Path, frames_per_second: float, **kwargs):
    """
    Makes a .gif which shows many images at a given frame rate.
    All images should be in order (don't know how this works) in the image directory

    Only tested with .png images but may work with others.

    :param image_directory:
    :type image_directory: pathlib.Path
    :param frames_per_second:
    :type frames_per_second: float
    :param kwargs: image_type='png' or other
    :return: nothing
    """
    assert isinstance(image_directory, pathlib.Path), "input must be a pathlib object"
    image_type = kwargs.get('type', 'png')

    timestampStr = datetime.now().strftime("%y%m%d_%H%M%S")
    gif_dir = image_directory.joinpath(timestampStr + "_GIF.gif")

    print('Started making GIF')
    print('Please wait... ')

    images = []
    for file_name in image_directory.glob('*.' + image_type):
        images.append(imageio.imread(image_directory.joinpath(file_name)))
    imageio.mimsave(gif_dir.as_posix(), images, fps=frames_per_second)

    print('Finished making GIF!')
    print('GIF can be found at: ' + gif_dir.as_posix())


def main():
    fps = 2
    png_dir = pathlib.Path('C:/temp/my_images')
    make_gif(png_dir, fps)

if __name__ == "__main__":
    main()