使用Swift 3并采用最快的黑客方法，您可以使用扩展来改进代码:

extension UITableViewCell {

    var isSeparatorHidden: Bool {
        get {
            return self.separatorInset.right != 0
        }
        set {
            if newValue {
                self.separatorInset = UIEdgeInsetsMake(0, self.bounds.size.width, 0, 0)
            } else {
                self.separatorInset = UIEdgeInsetsMake(0, 0, 0, 0)
            }
        }
    }

}

然后，当你配置单元格时:

func tableView(_ tableView: UITableView, cellForRowAt indexPath: IndexPath) -> UITableViewCell {
    let cell = collectionView.dequeueReusableCell(withReuseIdentifier: "identifier", for: indexPath)
    switch indexPath.row {
       case 3:
          cell.isSeparatorHidden = true
       default:
          cell.isSeparatorHidden = false
    }
    return cell
}

func tableView(_ tableView: UITableView, didSelectRowAt indexPath: IndexPath) {
    let cell = tableView.cellForRow(at: indexPath)
    if cell.isSeparatorHidden { 
       // do stuff
    }
}

使用这个子类，set separatorInset在iOS 9.2.1中不起作用，内容会被压缩。

@interface NSPZeroMarginCell : UITableViewCell

@property (nonatomic, assign) BOOL separatorHidden;

@end

@implementation NSPZeroMarginCell

- (void) layoutSubviews {
    [super layoutSubviews];

    for (UIView *view in  self.subviews) {
        if (![view isKindOfClass:[UIControl class]]) {
            if (CGRectGetHeight(view.frame) < 3) {
                view.hidden = self.separatorHidden;
            }
        }
    }
}

@end

https://gist.github.com/liruqi/9a5add4669e8d9cd3ee9

如果你不想自己画分隔符，使用这个:

  // Hide the cell separator by moving it to the far right
  cell.separatorInset = UIEdgeInsetsMake(0, 10000, 0, 0);

这个API只能从ios7开始使用。

迅速:

public func tableView(_ tableView: UITableView, cellForRowAt indexPath: IndexPath) -> UITableViewCell {

    ...

    // remove separator for last cell
    cell.separatorInset = indexPath.row < numberOfRowsInSection-1
        ? tableView.separatorInset
        : UIEdgeInsets(top: 0, left: tableView.bounds.size.width, bottom: 0, right: 0)

    return cell
}

objective - c:

- (UITableViewCell *)tableView:(UITableView *)tableView
     cellForRowAtIndexPath:(NSIndexPath *)indexPath {

    ...

    // remove separator for last cell
    cell.separatorInset = (indexPath.row < numberOfRowsInSection-1)
        ? tableView.separatorInset
        : UIEdgeInsetsMake(0.f, tableView.bounds.size.width, 0.f, 0.f);

    return cell;
}

我的开发环境是

Xcode 7.0 7A220 Swift 2.0 iOS 9.0

以上答案并不完全适用于我

经过尝试，我最终的工作解决方案是:

let indent_large_enought_to_hidden:CGFloat = 10000
cell.separatorInset = UIEdgeInsetsMake(0, indent_large_enought_to_hidden, 0, 0) // indent large engough for separator(including cell' content) to hidden separator
cell.indentationWidth = indent_large_enought_to_hidden * -1 // adjust the cell's content to show normally
cell.indentationLevel = 1 // must add this, otherwise default is 0, now actual indentation = indentationWidth * indentationLevel = 10000 * 1 = -10000

结果是:

在tableview单元格类中。放这行代码

separatorInset = UIEdgeInsets(top: 0, left: 0, bottom: 0, right: self.bounds.size.width)