我也有同样的问题。取消这个复选框。因为您是在代码中设置内容大小。

@Matteo Gobbi的答案是完美的，但在我的情况下，滚动视图不能滚动，我删除“对齐中心Y”，并添加“高度>=1”，滚动视图将成为可滚动的

如果你注意到滚动条在右边滚动，但内容没有移动，那么这个事实可能值得考虑:

您还可以在滚动视图的内容和滚动视图之外的对象之间使用约束，为滚动视图的内容提供固定位置，使该内容看起来漂浮在滚动视图之上。

这是苹果公司的文档。例如，如果你不小心把你的顶部标签/按钮/Img/视图钉在滚动区域之外的视图(也许是一个标题或scrollView上面的东西?)而不是contentView，你会冻结你的整个contentView在适当的位置。

在Interface Builder的大小检查器中设置ViewController(持有UIScrollView的ViewController)的大小为Freeform，所有这些都应该工作。

在界面构建器的大小检查器中为包含UIViewcontroller的自由形式设置

Swift 4+方法:

1)设置UIScrollView的上、下、左、右边缘为0

2)在UIScrollView中添加一个UIView，并设置顶部、底部、前边距和尾随边距为0(等于UIScrollView的边距)。

3)最重要的部分是设置宽度和高度，其中高度约束的优先级应该低。

private func setupConstraints() {

    // Constraints for scrollView
    scrollView.topAnchor.constraint(equalTo: view.topAnchor).isActive = true
    scrollView.leadingAnchor.constraint(equalTo: view.leadingAnchor).isActive = true
    scrollView.trailingAnchor.constraint(equalTo: view.trailingAnchor).isActive = true
    scrollView.bottomAnchor.constraint(equalTo: view.bottomAnchor).isActive = true

    // Constraints for containerView
    containerView.topAnchor.constraint(equalTo: scrollView.topAnchor).isActive = true
    containerView.bottomAnchor.constraint(equalTo: scrollView.bottomAnchor).isActive = true
    containerView.leadingAnchor.constraint(equalTo: scrollView.leadingAnchor).isActive = true
    containerView.trailingAnchor.constraint(equalTo: scrollView.trailingAnchor).isActive = true
    containerView.widthAnchor.constraint(equalTo: scrollView.widthAnchor).isActive = true

    let heightConstraint = containerView.heightAnchor.constraint(equalTo: scrollView.heightAnchor)
    heightConstraint.priority = UILayoutPriority(rawValue: 250)
    heightConstraint.isActive = true
}

This error took me a while to track down, initially I tried pinning the ScrollView's size but the error message clearly says "content size". I made sure everything I pinned from the top of the ScrollView was also pinned to the bottom. That way the ScrollView can calculate its content height by finding the height of all the objects & constraints. This solved the ambiguous content height, the width is pretty similar... Initially I had most things X centered in the ScrollView, but I had to also pin the objects to the side of the ScrollView. I don't like this because the iPhone6 might have a wider screen but it will remove the 'ambiguous content width' error.