你可以使用下面的代码:

 int DateDifInSecond = EndDate.Subtract(StartDate).TotalSeconds

我认为这会达到你的目的:

DateTime d1 = DateTime.Now;
DateTime d2 = DateTime.Now.AddDays(-1);

TimeSpan t = d1 - d2;
double NrOfDays = t.TotalDays;

首先声明一个稍后会返回的类:

public void date()
{
    Datetime startdate;
    Datetime enddate;
    Timespan remaindate;

    startdate = DateTime.Parse(txtstartdate.Text).Date;
    enddate = DateTime.Parse(txtenddate.Text).Date;

    remaindate = enddate - startdate;

    if (remaindate != null)
    {
        lblmsg.Text = "you have left with " + remaindate.TotalDays + "days.";
    }
    else
    {
        lblmsg.Text = "correct your code again.";
    }
}

protected void btncal_Click(object sender, EventArgs e)
{
    date();
}

使用按钮控件调用上面的类。这里有一个例子:

DateTime xmas = new DateTime(2009, 12, 25);
double daysUntilChristmas = xmas.Subtract(DateTime.Today).TotalDays;

date格式为"dd/MM/yyyy"

  string[] d1 = txtFromDate.Values.Split('/');
  string[] d2 = txtToDate.Values.Split('/');

  DateTime FrmDt = new DateTime(Convert.ToInt32(d1[2]), Convert.ToInt32(d1[1]), Convert.ToInt32(d1[0]));
  DateTime ToDt = new DateTime(Convert.ToInt32(d2[2]), Convert.ToInt32(d2[1]), Convert.ToInt32(d2[0]));

  TimeSpan TDiff = ToDt.Subtract(FrmDt);
  String DaysDiff = TDiff.TotalDays.ToString();

protected void Calendar1_SelectionChanged(object sender, EventArgs e)
{
    DateTime d = Calendar1.SelectedDate;
    // int a;
    TextBox2.Text = d.ToShortDateString();
    string s = Convert.ToDateTime(TextBox2.Text).ToShortDateString();
    string s1 =  Convert.ToDateTime(Label7.Text).ToShortDateString();
    DateTime dt = Convert.ToDateTime(s).Date;
    DateTime dt1 = Convert.ToDateTime(s1).Date;
    if (dt <= dt1)
    {
        Response.Write("<script>alert(' Not a valid Date to extend warranty')</script>");
    }
    else
    {
        string diff = dt.Subtract(dt1).ToString();
        Response.Write(diff);
        Label18.Text = diff;
        Session["diff"] = Label18.Text;
    }
}