ByteArrayOutputStream out = new ByteArrayOutputStream();
byte[] buffer = new byte[1024];
while (true) {
    int r = in.read(buffer);
    if (r == -1) break;
    out.write(buffer, 0, r);
}

byte[] ret = out.toByteArray();

public static byte[] getBytesFromInputStream(InputStream is) throws IOException {
    ByteArrayOutputStream os = new ByteArrayOutputStream(); 
    byte[] buffer = new byte[0xFFFF];
    for (int len = is.read(buffer); len != -1; len = is.read(buffer)) { 
        os.write(buffer, 0, len);
    }
    return os.toByteArray();
}

下面是一个优化的版本，尽量避免复制数据字节:

private static byte[] loadStream (InputStream stream) throws IOException {
   int available = stream.available();
   int expectedSize = available > 0 ? available : -1;
   return loadStream(stream, expectedSize);
}

private static byte[] loadStream (InputStream stream, int expectedSize) throws IOException {
   int basicBufferSize = 0x4000;
   int initialBufferSize = (expectedSize >= 0) ? expectedSize : basicBufferSize;
   byte[] buf = new byte[initialBufferSize];
   int pos = 0;
   while (true) {
      if (pos == buf.length) {
         int readAhead = -1;
         if (pos == expectedSize) {
            readAhead = stream.read();       // test whether EOF is at expectedSize
            if (readAhead == -1) {
               return buf;
            }
         }
         int newBufferSize = Math.max(2 * buf.length, basicBufferSize);
         buf = Arrays.copyOf(buf, newBufferSize);
         if (readAhead != -1) {
            buf[pos++] = (byte)readAhead;
         }
      }
      int len = stream.read(buf, pos, buf.length - pos);
      if (len < 0) {
         return Arrays.copyOf(buf, pos);
      }
      pos += len;
   }
}

你可以完全避免缓冲。

从http://www.exampledepot.com/egs/java.io/File2ByteArray.html复制的代码(是的，它非常冗长，但需要的内存大小是其他解决方案的一半)。

// Returns the contents of the file in a byte array.
public static byte[] getBytesFromFile(File file) throws IOException {
    InputStream is = new FileInputStream(file);

    // Get the size of the file
    long length = file.length();

    // You cannot create an array using a long type.
    // It needs to be an int type.
    // Before converting to an int type, check
    // to ensure that file is not larger than Integer.MAX_VALUE.
    if (length > Integer.MAX_VALUE) {
        // File is too large
    }

    // Create the byte array to hold the data
    byte[] bytes = new byte[(int)length];

    // Read in the bytes
    int offset = 0;
    int numRead = 0;
    while (offset < bytes.length
           && (numRead=is.read(bytes, offset, bytes.length-offset)) >= 0) {
        offset += numRead;
    }

    // Ensure all the bytes have been read in
    if (offset < bytes.length) {
        throw new IOException("Could not completely read file "+file.getName());
    }

    // Close the input stream and return bytes
    is.close();
    return bytes;
}

和往常一样，Spring框架(Spring core从3.2.2开始)也为你提供了一些东西:

Java 8方式(感谢BufferedReader和Adam Bien)

private static byte[] readFully(InputStream input) throws IOException {
    try (BufferedReader buffer = new BufferedReader(new InputStreamReader(input))) {
        return buffer.lines().collect(Collectors.joining("\n")).getBytes(<charset_can_be_specified>);
    }
}

注意，这个解决方案删除回车符('\r')，可能是不合适的。