使用普通Java的DataInputStream和它的readFully方法(至少从Java 1.4开始存在):

...
byte[] bytes = new byte[(int) file.length()];
DataInputStream dis = new DataInputStream(new FileInputStream(file));
dis.readFully(bytes);
...

这个方法还有其他一些形式，但在这个用例中，我一直使用这个方法。

你可以试试仙人掌:

byte[] array = new BytesOf(stream).bytes();

Java 7及以上版本:

import sun.misc.IOUtils;
...
InputStream in = ...;
byte[] buf = IOUtils.readFully(in, -1, false);

你可以完全避免缓冲。

从http://www.exampledepot.com/egs/java.io/File2ByteArray.html复制的代码(是的，它非常冗长，但需要的内存大小是其他解决方案的一半)。

// Returns the contents of the file in a byte array.
public static byte[] getBytesFromFile(File file) throws IOException {
    InputStream is = new FileInputStream(file);

    // Get the size of the file
    long length = file.length();

    // You cannot create an array using a long type.
    // It needs to be an int type.
    // Before converting to an int type, check
    // to ensure that file is not larger than Integer.MAX_VALUE.
    if (length > Integer.MAX_VALUE) {
        // File is too large
    }

    // Create the byte array to hold the data
    byte[] bytes = new byte[(int)length];

    // Read in the bytes
    int offset = 0;
    int numRead = 0;
    while (offset < bytes.length
           && (numRead=is.read(bytes, offset, bytes.length-offset)) >= 0) {
        offset += numRead;
    }

    // Ensure all the bytes have been read in
    if (offset < bytes.length) {
        throw new IOException("Could not completely read file "+file.getName());
    }

    // Close the input stream and return bytes
    is.close();
    return bytes;
}

我知道已经太迟了，但我认为这里有更清晰的解决方案，更易于阅读……

/**
 * method converts {@link InputStream} Object into byte[] array.
 * 
 * @param stream the {@link InputStream} Object.
 * @return the byte[] array representation of received {@link InputStream} Object.
 * @throws IOException if an error occurs.
 */
public static byte[] streamToByteArray(InputStream stream) throws IOException {

    byte[] buffer = new byte[1024];
    ByteArrayOutputStream os = new ByteArrayOutputStream();

    int line = 0;
    // read bytes from stream, and store them in buffer
    while ((line = stream.read(buffer)) != -1) {
        // Writes bytes from byte array (buffer) into output stream.
        os.write(buffer, 0, line);
    }
    stream.close();
    os.flush();
    os.close();
    return os.toByteArray();
}

我试图编辑@numan的答案，修复了写垃圾数据，但编辑被拒绝。虽然这段简短的代码并不出色，但我看不到其他更好的答案。以下是我认为最有意义的建议:

ByteArrayOutputStream out = new ByteArrayOutputStream();
byte[] buffer = new byte[1024]; // you can configure the buffer size
int length;

while ((length = in.read(buffer)) != -1) out.write(buffer, 0, length); //copy streams
in.close(); // call this in a finally block

byte[] result = out.toByteArray();

ByteArrayOutputStream不需要关闭。为了可读性，省略了一些结构