你可以用:

with open('data.txt', 'r') as file:
    data = file.read().replace('\n', '')

或者文件内容保证为一行

with open('data.txt', 'r') as file:
    data = file.read().rstrip()

with open("data.txt") as myfile:
    data="".join(line.rstrip() for line in myfile)

Join()将连接一个字符串列表，而不带参数的rstrip()将从字符串末尾删除空格，包括换行符。

如此: 将文件更改为:

LLKKKKKKKKMMMMMMMMNNNNNNNNNNNNN GGGGGGGGGHHHHHHHHHHHHHHHHHHHHEEEEEEEE

然后:

file = open("file.txt")
line = file.read()
words = line.split()

这将创建一个名为words的列表，等于:

['LLKKKKKKKKMMMMMMMMNNNNNNNNNNNNN', 'GGGGGGGGGHHHHHHHHHHHHHHHHHHHHEEEEEEEE']

去掉了"\n"。要回答括号阻碍你的问题，只需这样做:

for word in words: # Assuming words is the list above
    print word # Prints each word in file on a different line

Or:

print words[0] + ",", words[1] # Note that the "+" symbol indicates no spaces
#The comma not in parentheses indicates a space

这将返回:

LLKKKKKKKKMMMMMMMMNNNNNNNNNNNNN, GGGGGGGGGHHHHHHHHHHHHHHHHHHHHEEEEEEEE

也许你可以试试这个?我在我的程序中使用这个。

Data= open ('data.txt', 'r')
data = Data.readlines()
for i in range(len(data)):
    data[i] = data[i].strip()+ ' '
data = ''.join(data).strip()

这里有四个代码供您选择:

with open("my_text_file.txt", "r") as file:
    data = file.read().replace("\n", "")

or

with open("my_text_file.txt", "r") as file:
    data = "".join(file.read().split("\n"))

or

with open("my_text_file.txt", "r") as file:
    data = "".join(file.splitlines())

or

with open("my_text_file.txt", "r") as file:
    data = "".join([line for line in file])

file = open("myfile.txt", "r")
lines = file.readlines()
str = ''                                     #string declaration

for i in range(len(lines)):
    str += lines[i].rstrip('\n') + ' '

print str